Artículos de revistas
Surface-modified bioresorbable electrospun scaffolds for improving hemocompatibility of vascular grafts
Fecha
2017-03Registro en:
Caracciolo, Pablo Christian; Rial-Hermida, María Isabel; Montini Ballarin, Florencia; Abraham, Gustavo Abel; Concheiro, Angel; et al.; Surface-modified bioresorbable electrospun scaffolds for improving hemocompatibility of vascular grafts; Elsevier Science; Materials Science and Engineering: C; 75; 3-2017; 1115-1127
0928-4931
CONICET Digital
CONICET
Autor
Caracciolo, Pablo Christian
Rial-Hermida, María Isabel
Montini Ballarin, Florencia
Abraham, Gustavo Abel
Concheiro, Angel
Álvarez Lorenzo, Carmen
Resumen
The replacement of small-diameter vessels is one of the main challenges in tissue engineering. Moreover, the surface modification of small-diameter vascular grafts (SDVG) is a key factor in the success of the therapy due to their increased thrombogenicity and infection susceptibility caused by the lack of a functional endothelium.In this work, electrospun scaffolds were prepared from blends of poly(L-lactic acid) (PLLA) and segmented polyurethane (PHD) with a composition designed to perform as SDVG inner layer. The scaffolds were then successfully surface-modified with heparin following two different strategies that rely on grafting of heparin to eitherPLLA or PHD functional groups. Both strategies afforded high heparin density, being higher for urethane methodology. The functionalized scaffolds did not cause hemolysis and inhibited platelet adhesion to a large extent. However, lysozyme/heparin-functionalized scaffolds obtained through urethane methodology achieved the highest platelet attachment inhibition. The increase in hydrophilicity and water absorption of the surfacefunctionalized nanostructures favored adhesion and proliferation of human adipose-derived stemcells. Heparinized surfaces conjugated with lysozyme presented microbial hydrolysis activity dependent on heparin content. Overall, a better performance obtained for urethane-modified scaffold, added to the fact that no chain scission is involved in urethane methodology, makes the latter the best choice for surface modification of PLLA/PHD 50/50 electrospun scaffolds. Scaffolds functionalized by this route may perform as advanced components ofSDVG suitable for vascular tissue engineering, exhibiting biomimetic behavior, avoiding thrombi formation and providing antimicrobial features.