dc.creator | Rubio, Andrea E. | |
dc.creator | Abraha, Awet | |
dc.creator | Carpenter, Crystal A. | |
dc.creator | Troyer, Ryan M. | |
dc.creator | Reyes Rodríguez, Ángel L. | |
dc.creator | Salomon, Horacio Eduardo | |
dc.creator | Arts, Eric J. | |
dc.creator | Tebit, Denis M. | |
dc.date.accessioned | 2017-12-11T20:42:08Z | |
dc.date.accessioned | 2018-11-06T13:50:36Z | |
dc.date.available | 2017-12-11T20:42:08Z | |
dc.date.available | 2018-11-06T13:50:36Z | |
dc.date.created | 2017-12-11T20:42:08Z | |
dc.date.issued | 2014-04 | |
dc.identifier | Rubio, Andrea E.; Abraha, Awet; Carpenter, Crystal A.; Troyer, Ryan M.; Reyes Rodríguez, Ángel L.; et al.; Similar Replicative Fitness Is Shared by the Subtype B and Unique BF Recombinant HIV-1 Isolates that Dominate the Epidemic in Argentina; Public Library of Science; Plos One; 9; 4; 4-2014; 1-15; e92084 | |
dc.identifier | 1932-6203 | |
dc.identifier | http://hdl.handle.net/11336/30199 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1880028 | |
dc.description.abstract | The HIV-1 epidemic in South America is dominated by pure subtypes (mostly B and C) and more than 7 BF and BC recombinant forms. In Argentina, circulating recombinant forms (CRFs) comprised of subtypes B and F make up more than 50% of HIV infections. For this study, 28 HIV-1 primary isolates were obtained from patients in Buenos Aires, Argentina and initially classified into subtype B (n = 9, 32.1%), C (n = 1, 3.6%), and CRFs (n = 18, 64.3%) using partial pol and vpu-env sequences, which proved to be inconsistent and inaccurate for these phylogenetic analyses. Near full length genome sequences of these primary HIV-1 isolates revealed that nearly all intersubtype BF recombination sites were unique and countered previous “CRF” B/F classifications. The majority of these Argentinean HIV-1 isolates were CCR5-using but 4 had a dual/mixed tropism as predicted by both phenotypic and genotypic assays. Comparison of the replicative fitness of these BF primary HIV-1 isolates to circulating B, F, and C HIV-1 using pairwise competitions in peripheral blood mononuclear cells (PBMCs) indicated a similarity in fitness of these BF recombinants to subtypes B and F HIV-1 (of the same co-receptor usage) whereas subtype C HIV-1 was significantly less fit than all as previously reported. These results suggest that the multitude of BF HIV-1 strains present within the Argentinean population do not appear to have gained replicative fitness following recent B and F recombination events. | |
dc.language | eng | |
dc.publisher | Public Library of Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0092084 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092084 | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | HIV | |
dc.subject | FITNESS | |
dc.subject | SUBTYPE B | |
dc.subject | BF RECOMBINANT | |
dc.title | Similar Replicative Fitness Is Shared by the Subtype B and Unique BF Recombinant HIV-1 Isolates that Dominate the Epidemic in Argentina | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |