Artículos de revistas
ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England
Fecha
2010-08Registro en:
Dinolfo, María Inés; Stenglein, Sebastian Alberto; Moreno, Maria Virginia; Nicholson, Paul; Jennings, Philip; et al.; ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England; Springer; European Journal Of Plant Pathology; 127; 4; 8-2010; 483-491
0929-1873
1573-8469
Autor
Dinolfo, María Inés
Stenglein, Sebastian Alberto
Moreno, Maria Virginia
Nicholson, Paul
Jennings, Philip
Salerno, Graciela Lidia
Resumen
Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.