Artículos de revistas
Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study
Fecha
2003-12Registro en:
Rivas, Maria Gabriela; Gennaro, Ana Maria; Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study; Elsevier Ireland; Chemistry And Physics Of Lipids; 122; 12-2003; 165-169
0009-3084
CONICET Digital
CONICET
Autor
Rivas, Maria Gabriela
Gennaro, Ana Maria
Resumen
We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors. However, the protein profile of these samples showed important differences with that of DRM from untreated cells. The analysis of our results suggests that the effect of Triton X-100 on cholesterol depleted erythrocytes is limited to the solubilization of raft proteins, without disrupting the lipid matrix of DRM.