info:eu-repo/semantics/article
A role for the endocannabinoid system in premature luteal regression and progesterone withdrawal in lipopolysaccharide-induced early pregnancy loss model
Fecha
2016-11Registro en:
Schander, Julieta Aylen; Correa, Fernando Gabriel; Bariani, Maria Victoria; Blanco, Julieta; Cymeryng, Cora Betriz; et al.; A role for the endocannabinoid system in premature luteal regression and progesterone withdrawal in lipopolysaccharide-induced early pregnancy loss model; Oxford University Press; Molecular Human Reproduction; 22; 11; 11-2016; 800-808
1360-9947
CONICET Digital
CONICET
Autor
Schander, Julieta Aylen
Correa, Fernando Gabriel
Bariani, Maria Victoria
Blanco, Julieta
Cymeryng, Cora Betriz
Jensen, Cristian Federico
Wolfson, Manuel Luis
Franchi, Ana Maria
Resumen
Appropriate systemic progesterone levels are critical for a successful pregnancy outcome. Precocious loss of luteal progesterone secretion leads to miscarriage in rodents. We have previously shown that lipopolysaccharide (LPS) administration to pregnant mice induces embryonic resorption accompanied by a dramatic decrease in systemic progesterone levels in a murine model of inflammatory miscarriage. Furthermore, we showed that the endocannabinoid system (eCS) mediated LPS deleterious effects. The aim of this study was to explore the participation of the endocrine system in the LPS-induced miscarriage as well as the role of the eCS in this process. We found that LPS increased the expression of COX-2 and the production of PGF2α in the uterus of 7-days pregnant mice. Increased production of PGF2α resulted in a lower expression of prolactin receptor in the ovary and a marked regression of corpora lutea (CL), which thus produces less progesterone and consequently, results in embryo resorption. Remarkably, these effects were completely absent in CB1-knockout pregnant mice. Our results clearly suggest that the absence of CB1 receptor confers resistance to LPS deleterious actions during pregnancy. Moreover, lack of CB1 receptor protected from LPS-induced premature luteal regression.