dc.creator | de Campos Nebel, Ildefonso Marcelo | |
dc.creator | Palmitelli, Micaela | |
dc.creator | Gonzalez Cid, Marcela Beatriz | |
dc.date.accessioned | 2018-05-14T01:04:59Z | |
dc.date.accessioned | 2018-11-06T13:25:59Z | |
dc.date.available | 2018-05-14T01:04:59Z | |
dc.date.available | 2018-11-06T13:25:59Z | |
dc.date.created | 2018-05-14T01:04:59Z | |
dc.date.issued | 2017-07 | |
dc.identifier | de Campos Nebel, Ildefonso Marcelo; Palmitelli, Micaela; Gonzalez Cid, Marcela Beatriz; Measurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry; Wiley; Current protocols in cytometry; 81; 7; 7-2017; 7.48.1-7.48.8 | |
dc.identifier | 1934-9297 | |
dc.identifier | http://hdl.handle.net/11336/45036 | |
dc.identifier | 1934-9300 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1875282 | |
dc.description.abstract | The poisoning of Topoisomerase II (Top2) has been found to be useful as a therapeutic strategy for the treatment of several tumors. The mechanisms of these agents involves a drug-mediated stabilization of a Top2-DNA complex, termed Top2 cleavage complex (Top2cc), which maintains a 5´ end of DNA covalently bound to a tyrosine from Top2 through a phosphodiester group. Drug-stabilized Top2cc leads to Top2 linked-DNA breaks which are believed to mediate their cytotoxicity. Several time-consuming or cell type-limiting assays have been used in the past to study drug-stabilized Top2cc. Here, we describe a flow cytometry-based method that allows arapid assessment of drug-induced Top2cc, which is suitable for high throughput analysis in almost any kind of human cell. The analyses of the drug-induced Top2cc in the cell cycle context and the possibility to track its removal are additional benefits from this methodology. | |
dc.language | eng | |
dc.publisher | Wiley | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpcy.21 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cpcy.21 | |
dc.relation | info:eu-repo/semantics/altIdentifier/pmid/28678420 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | TOPOISOMERASE II | |
dc.subject | CLEAVAGE COMPLEXES | |
dc.subject | HIGH-THROUGHPUT ANALYSIS | |
dc.subject | HUMAN CELLS | |
dc.subject | DNA TOPOISOMERASES | |
dc.subject | CITOMETRY | |
dc.title | Measurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |