dc.creatorde Campos Nebel, Ildefonso Marcelo
dc.creatorPalmitelli, Micaela
dc.creatorGonzalez Cid, Marcela Beatriz
dc.date.accessioned2018-05-14T01:04:59Z
dc.date.accessioned2018-11-06T13:25:59Z
dc.date.available2018-05-14T01:04:59Z
dc.date.available2018-11-06T13:25:59Z
dc.date.created2018-05-14T01:04:59Z
dc.date.issued2017-07
dc.identifierde Campos Nebel, Ildefonso Marcelo; Palmitelli, Micaela; Gonzalez Cid, Marcela Beatriz; Measurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry; Wiley; Current protocols in cytometry; 81; 7; 7-2017; 7.48.1-7.48.8
dc.identifier1934-9297
dc.identifierhttp://hdl.handle.net/11336/45036
dc.identifier1934-9300
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1875282
dc.description.abstractThe poisoning of Topoisomerase II (Top2) has been found to be useful as a therapeutic strategy for the treatment of several tumors. The mechanisms of these agents involves a drug-mediated stabilization of a Top2-DNA complex, termed Top2 cleavage complex (Top2cc), which maintains a 5´ end of DNA covalently bound to a tyrosine from Top2 through a phosphodiester group. Drug-stabilized Top2cc leads to Top2 linked-DNA breaks which are believed to mediate their cytotoxicity. Several time-consuming or cell type-limiting assays have been used in the past to study drug-stabilized Top2cc. Here, we describe a flow cytometry-based method that allows arapid assessment of drug-induced Top2cc, which is suitable for high throughput analysis in almost any kind of human cell. The analyses of the drug-induced Top2cc in the cell cycle context and the possibility to track its removal are additional benefits from this methodology.
dc.languageeng
dc.publisherWiley
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpcy.21
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cpcy.21
dc.relationinfo:eu-repo/semantics/altIdentifier/pmid/28678420
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectTOPOISOMERASE II
dc.subjectCLEAVAGE COMPLEXES
dc.subjectHIGH-THROUGHPUT ANALYSIS
dc.subjectHUMAN CELLS
dc.subjectDNA TOPOISOMERASES
dc.subjectCITOMETRY
dc.titleMeasurement of drug-stabilized Topoisomerase II cleavage complexes by Flow Cytometry
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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