info:eu-repo/semantics/article
Contribution of resident and recruited macrophages to the photodynamic intervention of colorectal tumor microenvironment
Fecha
2016-01Registro en:
Pansa, Maria Florencia; Lamberti, María Julia; Cogno, Ingrid Sol; Correa, Silvia Graciela; Rumie Vittar, Natalia Belen; et al.; Contribution of resident and recruited macrophages to the photodynamic intervention of colorectal tumor microenvironment; Karger; Tumor Biology; 37; 1; 1-2016; 541-552
1010-4283
1423-0380
CONICET Digital
CONICET
Autor
Pansa, Maria Florencia
Lamberti, María Julia
Cogno, Ingrid Sol
Correa, Silvia Graciela
Rumie Vittar, Natalia Belen
Rivarola, Viviana
Resumen
The study of cellular interactions in the tumor microenvironment has become one of the main areas of research in the fight against cancer. Tumor-associated macrophages (TAMs) influence tumor progression and therapy response due to its functional plasticity. Regarding cancer treatment, photodynamic therapy (PDT) is a minimally invasive and clinically approved procedure that involves the administration of a photosensitizer (PS), a nontoxic photosensitizing drug which is selectively retained in neoplastic tissue. Here, we investigated the role of resident and nonresident macrophages in the context of a PDT-treated colorectal tumor by developing a combination of 2-D and three-dimensional (3-D) experimental platform, recreating tumor-stroma interactions in vitro. Enhancement of cytotoxicity of PDT was achieved in the presence of nonresident macrophages which had a strong anti-tumor phenotype mediated by the production of nitric oxide, IL-6, and tumor necrosis factor alpha (TNF-α). On the contrary, tumor resident macrophages induced a pro-tumor phenotype promoting tumor cell migration and endothelial stimulation. Due to their plasticity, tumor-resident or tumor-recruited macrophages can differentially influence the response of tumors to PDT, so their multifactorial roles should be considered in the overall design of anti-tumor therapeutic.