Artículos de revistas
Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function
Fecha
2012-08Registro en:
Manchado Rojo, María; Delgado Benarroch, Luciana; Roca, María José; Weiss, Julia; Marcos, Egea Cortines; Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function; Wiley Blackwell Publishing, Inc; Plant Journal; 72; 2; 8-2012; 294–307
0960-7412
1365-313X
CONICET Digital
CONICET
Autor
Manchado Rojo, María
Delgado Benarroch, Luciana
Roca, María José
Weiss, Julia
Marcos, Egea Cortines
Resumen
The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def nicotianoides (def nic), and absent in co def nic double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def nic and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits<br />appear early in development.