dc.creatorSoto, Gabriela Cynthia
dc.creatorFox, Ana Romina
dc.creatorAyub, Nicolás Daniel
dc.date.accessioned2017-09-21T20:32:28Z
dc.date.accessioned2018-11-06T12:56:45Z
dc.date.available2017-09-21T20:32:28Z
dc.date.available2018-11-06T12:56:45Z
dc.date.created2017-09-21T20:32:28Z
dc.date.issued2013-08
dc.identifierSoto, Gabriela Cynthia; Fox, Ana Romina; Ayub, Nicolás Daniel; Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes; Springer; Journal Of Molecular Evolution; 77; 1-2; 8-2013; 3-7
dc.identifier0022-2844
dc.identifierhttp://hdl.handle.net/11336/24861
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1871483
dc.description.abstractBiological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00239-013-9578-8
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectNITROGEN FIXATION
dc.subjectEVOLUTION
dc.subjectLATERAL TRANSFER
dc.subjectBACTERIA
dc.subjectEUKARYOTES
dc.subjectOXYGEN
dc.titleExploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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