info:eu-repo/semantics/article
A new semisynthetic derivative of sauroine induces LTP in hippocampal slices and improves learning perfomance in the Morris Water Maze
Fecha
2014-03Registro en:
Vallejo, Mariana Guadalupe; Loyola, S.; Contreras, Darwin; Ugarte, Gonzalo; Cifuente, Diego Alberto; et al.; A new semisynthetic derivative of sauroine induces LTP in hippocampal slices and improves learning perfomance in the Morris Water Maze; Wiley; Journal of Neurochemistry; 129; 5; 3-2014; 864-876
0022-3042
Autor
Vallejo, Mariana Guadalupe
Loyola, S.
Contreras, Darwin
Ugarte, Gonzalo
Cifuente, Diego Alberto
Ortega, María Gabriela
Cabrera, Jose Luis
Zeise, Marc
Tonn, Carlos Eugenio
Carreño, Mario
Delgado, Ricardo
Morales, Bernardo
Agnese, Alicia Mariel
Resumen
Two semisynthetic acetyl derivatives of the alkaloid sauroine from Huperzia saururus, monoacetyl sauroine, and diacetyl sauroine (DAS) were obtained and their chemical structures were analyzed by NMR. While monoacetyl sauroine is the typical product of acetylation, DAS is an unexpected derivative related to the keto-enol formation of sauroine. Recordings of field excitatory post-synaptic potentials from the CA1 region of rat hippocampal slices showed that only DAS acutely applied induced chemical long-term potentiation (LTP) in a dose-dependent manner with an EC50 of 1.15 0.09 lM. This effect was blocked by 10 lM D(-)-2-amino-5-phosphonopentanoic acid (AP5), suggesting dependence on the NMDA receptor. DAS significantly increased NMDA receptor-dependent excitatory post-synaptic currents without affecting a-amino- 3-hydroxy-5-methylisoxazole-4-propionate receptor-dependent currents. Repetitive administration of DAS improved visuospatial learning in the Morris Water Maze. In slices from rats tested in the Morris Water Maze, LTP resulting from electrical synaptic stimulation was 2.5 times larger than in controls. Concentration of DAS measured in the brain after repetitive administration was 29.5 lM. We conclude that slices perfused with DAS display a robust NMDA receptor-dependent chemical LTP. During chronic treatment, DAS enhances