Artículos de revistas
Characterization of bovine MHC DRB3 diversity in Latin American Creole cattle breeds
Fecha
2013-04Registro en:
Giovambattista, Guillermo; Takeshima, Shin Nosuke; Ripoli, María Verónica; Matsumoto, Yuki; Alvarez Franco, Luz Angela; et al.; Characterization of bovine MHC DRB3 diversity in Latin American Creole cattle breeds; Elsevier Science; Gene; 519; 1; 4-2013; 150-158
0378-1119
Autor
Giovambattista, Guillermo
Takeshima, Shin Nosuke
Ripoli, María Verónica
Matsumoto, Yuki
Alvarez Franco, Luz Angela
Saito, Hideki
Onuma, Misao
Aida, Yoko
Resumen
In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for diseases and immunological traits. However, none of the highly adapted Latin American Creole breeds have been characterized for BoLA gene polymorphism by high resolution typing methods. In this work, we sequenced exon 2 of the BoLA class II DRB3 gene from 179 cattle (113 Bolivian Yacumeño cattle and 66 Colombian Hartón del Valle cattle breeds) using a polymerase chain reaction sequence-based typing (PCR-SBT) method. We identified 36 previously reported alleles and three novel alleles. Thirty-five (32 reported and three new) and 24 alleles (22 reported and two new) were detected in Yacumeño and Hartón del Valle breeds, respectively. Interestingly, Latin American Creole cattle showed a high degree of gene diversity despite their small population sizes, and 10 alleles including three new alleles were found only in these two Creole breeds. We next compared the degree of genetic variability at the population and sequence levels and the genetic distance in the two breeds with those previously reported in five other breeds: Holstein, Japanese Shorthorn, Japanese Black, Jersey, and Hanwoo. Both Creole breeds presented gene diversity higher than 0.90, a nucleotide diversity higher than 0.07, and mean number of pairwise differences higher than 19, indicating that Creole cattle had similar genetic diversity at BoLA-DRB3 to the other breeds. A neutrality test showed that the high degree of genetic variability may be maintained by balancing selection. The FST index and the exact G test showed significant differences across all cattle populations (FST = 0.0478; p < 0.001). Results from the principal components analysis and the phylogenetic tree showed that Yacumeño and Hartón del Valle breeds were closely related to each other. Collectively, our results suggest that the high level of genetic diversity could be explained by the multiple origins of the Creole germplasm (European, African and Indicus), and this diversity might be maintained by balancing selection.