info:eu-repo/semantics/article
Beneficial effects of hydroalcoholic extract and flavonoids from Zuccagnia punctata in a rabbit model of vascular dysfunction induced by high cholesterol diet
Fecha
2017-06Registro en:
Roco, Julieta; Alarcón, Gabriela del Jesús; Sierra, Liliana Beatríz; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Beneficial effects of hydroalcoholic extract and flavonoids from Zuccagnia punctata in a rabbit model of vascular dysfunction induced by high cholesterol diet; Birkhauser Boston Inc; Medicinal Chemistry Research; 26; 10; 6-2017; 2336-2344
1054-2523
CONICET Digital
CONICET
Autor
Roco, Julieta
Alarcón, Gabriela del Jesús
Sierra, Liliana Beatríz
Zampini, Iris Catiana
Isla, Maria Ines
Jerez, Susana Josefina
Resumen
Abstract This study evaluated the effects of a Zuccagniapunctata standardized hydroalcoholic extract (ZpE) and threeof its major flavonoids [2′,4′-dihydroxychalcone (DHC), 7-hydroxyflavanone (HF) and 3,7-dihydroxyflavone (DHF)] onthe vascular reactivity of aortic rings with endothelial dysfunctioninduced by feeding rabbits on a high cholesteroldiet. Rabbits were fed with either normal chow or a dietcontaining 1% cholesterol for 5?6 weeks. Isometric contractionswere measured. Concentration response curves to ZpE(range from 4×10−2 to 4 ×10 μg gallic acid equivalent/ml),DHC, DHF or HF (range from 10−9 to 10−4 M) showedconcentration-dependent relaxation of arteries pre-contractedwith phenylephrine. ZpE (4×10−2, 4 ×10−1 and 4 μg gallicacid equivalent/ml), HF (10−9, 10−7, 10−5 M), DHC (10−9M) and DHF (10−9 M) added to the bath improved acetylcholineaffinity. Pre-treatment of arteries with ZpE (4×10−2 μg gallic acid equivalent/ml) and DHC (10−9 M)reduced phenylephrine-induced contraction. Incubationwith the higher dose of ZpE (4 μg gallic acid equivalent/ml)reduced the angiotensin II-maximal contraction (Cmax) actingas a non-competitive antagonist, while DHC and DHF(10−5 M) caused a non-parallel rightward of the angiotensinII-concentration response curves and reduced the Cmaxacting as mixed antagonists. ZpE (4 × 10−2 μg gallic acidequivalent/ml), DHC and DHF (10−9 M) caused a rightwarddisplacement of angiotensin II-concentration responsecurves acting as competitive antagonists. In conclusion, thepresent study demonstrated that a ZpE and its major flavonoidshad beneficial effects in arteries with vasculardysfunction induced by hypercholesterolemia. Therefore itsuse as herbal medicine to prevent cardiovascular risks factorsmay be promising.