dc.creator | Falcón Dieguez, Jose | |
dc.creator | Rodi, Pablo Marcelo | |
dc.creator | Lores Guevara, Manuel | |
dc.creator | Gennaro, Ana Maria | |
dc.date.accessioned | 2017-03-06T18:20:32Z | |
dc.date.accessioned | 2018-11-06T12:34:06Z | |
dc.date.available | 2017-03-06T18:20:32Z | |
dc.date.available | 2018-11-06T12:34:06Z | |
dc.date.created | 2017-03-06T18:20:32Z | |
dc.date.issued | 2010-05 | |
dc.identifier | Falcón Dieguez, Jose; Rodi, Pablo Marcelo; Lores Guevara, Manuel; Gennaro, Ana Maria; Spin Label Studies of the Hemoglobin–Membrane Interaction During Sickle Hemoglobin Polymerization; Springer Wien; Applied Magnetic Resonance; 38; 4; 5-2010; 443-453 | |
dc.identifier | 0937-9347 | |
dc.identifier | http://hdl.handle.net/11336/13561 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1867901 | |
dc.description.abstract | An enhanced hemoglobin–membrane association has been previously documented in sickle cell anemia. However, it is not known how this interaction is modified during the hemoglobin S polymerization process. In this work, we use a model of reconstituted erythrocytes from ghost membranes whose cytoskeleton proteins had been previously labeled with the 4-maleimido Tempo spin label, and that were subsequently resealed with hemoglobin S or A solutions. Using electron paramagnetic resonance spectroscopy, we studied the time dependence of the spectral W/S parameter, indicative of the conformational state of cytoskeleton proteins (mainly spectrin) under spontaneous deoxygenation, with the aim of detecting the eventual effects due to hemoglobin S polymerization. The differences observed in the temporal behavior of W/S in erythrocytes reconstituted with both hemoglobins were considered as experimental evidence of an increment in hemoglobin S–membrane interaction as a result of the polymerization process of hemoglobin S under spontaneous deoxygenation. | |
dc.language | eng | |
dc.publisher | Springer Wien | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00723-010-0138-8 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | HEMOGLOBIN-MEMBRANE INTERACTION | |
dc.subject | HEMOGLOBIN S POLYMERIZATION | |
dc.subject | RECONSTITUTED ERYTHROCYTES | |
dc.subject | 4-MALEIMIDO TEMPO SPIN LABEL | |
dc.title | Spin Label Studies of the Hemoglobin–Membrane Interaction During Sickle Hemoglobin Polymerization | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |