dc.creatorPaez, Paulina Laura
dc.creatorBecerra, María Cecilia
dc.creatorAlbesa, Inés
dc.date.accessioned2017-04-03T20:16:47Z
dc.date.accessioned2018-11-06T12:26:10Z
dc.date.available2017-04-03T20:16:47Z
dc.date.available2018-11-06T12:26:10Z
dc.date.created2017-04-03T20:16:47Z
dc.date.issued2010-12
dc.identifierPaez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Antioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage; Wiley; Fundam. Clin. Pharmacol.; 24; 6; 12-2010; 771-776
dc.identifier0767-3981
dc.identifierhttp://hdl.handle.net/11336/14736
dc.identifier1472-8206
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1866575
dc.description.abstractThe aim of this investigation was to determine whether the antioxidant defences protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage. Reactive oxygen species (ROS) were determined by chemiluminescence and nitric oxide (NO) was assayed by Griess reaction. The accumulation of ciprofloxacin was examined by fluorometry and oxidation of protein, catalase, ferrous reduction antioxidant potency (FRAP), carbonyls and advanced oxidation protein products (AOPP), studied by spectrophotometry. Ciprofloxacin stimulated higher production of ROS and NO in the susceptible strains than in the resistant ones. There was higher accumulation of antibiotic in sensitive strains than in resistant ones, except for the most resistant strain, which accumulated an elevated amount of antibiotic. The FRAP/ciprofloxacin accumulation ratio of the antibiotic was lower in sensitive than in resistant strains. The most resistant strain exhibited the highest FRAP and presented a high catalase activity. There was oxidation of proteins in the presence of ciprofloxacin, with the carbonyl residues increasing in sensitive and resistant S. aureus. The degradation of carbonyls to AOPP in oxidized proteins was higher in the resistant than in sensitive strains. In conclusion, an increase in antioxidant capacity and a rapid oxidation of carbonyls to AOPP contributed to resistance to ciprofloxacin.
dc.languageeng
dc.publisherWiley
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1472-8206.2009.00806.x
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-8206.2009.00806.x/abstract
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectANTIBIOTICS
dc.subjectANTIOXIDANT CAPACITY
dc.subjectOXIDATIVE STRESS
dc.subjectPROTEIN OXIDATION
dc.titleAntioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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