Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves

dc.creatorMangano, Silvina
dc.creatorGonzalez, Cintia Daniela
dc.creatorPetruccelli, Silvana
dc.date.accessioned2018-01-05T16:15:23Z
dc.date.accessioned2018-11-06T12:24:37Z
dc.date.available2018-01-05T16:15:23Z
dc.date.available2018-11-06T12:24:37Z
dc.date.created2018-01-05T16:15:23Z
dc.date.issued2014-01
dc.identifierPetruccelli, Silvana; Gonzalez, Cintia Daniela; Mangano, Silvina; Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves; Humana Press; Methods In Molecular Biology (clifton, N.j.); 1062; 1-2014; 165-173
dc.identifier1064-3745
dc.identifierhttp://hdl.handle.net/11336/32397
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1866253
dc.description.abstractTransient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfi ltration is more rapid, and samples can be analyzed a few days after inocu-lation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalci-trant to routine transient assays. In this chapter, we describe a transient expression assay using simple infi ltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fl uorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium defi cient in nutrients and containing acetosyringone. Besides, Arabidopsisplants were cultivated in intermediate photoperiod (12 h light–12 h dark) to promote leaf growth.
dc.languageeng
dc.publisherHumana Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-62703-580-4_8
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-62703-580-4_8
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectTransient gene expression
dc.subjectArabidopsis thaliana
dc.subjectAgrobacterium tumefaciens
dc.subjectLeaf agroinfi ltration
dc.subjectFluorescent proteins
dc.titleAgrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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