Artículos de revistas
Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
Fecha
2017-01Registro en:
Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; et al.; Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens; Rockefeller University Press; Journal of Cell Biology; 216; 3; 1-2017; 1-11
0021-9525
CONICET Digital
CONICET
Autor
Valansi, Clari
Moi, David
Leikina, Evgenia
Matveev, Elena
Graña, Martín
Chernomordik, Leonid V.
Romero, Héctor
Aguilar, Pablo Sebastián
Podbilewicz, Benjamin
Resumen
Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.