dc.creator | Danielli, Mauro | |
dc.creator | Marinelli, Raul Alberto | |
dc.date.accessioned | 2018-07-17T14:37:56Z | |
dc.date.accessioned | 2018-11-06T12:15:52Z | |
dc.date.available | 2018-07-17T14:37:56Z | |
dc.date.available | 2018-11-06T12:15:52Z | |
dc.date.created | 2018-07-17T14:37:56Z | |
dc.date.issued | 2016-02 | |
dc.identifier | Danielli, Mauro; Marinelli, Raul Alberto; Lipid-based transfection reagents can interfere with cholesterol biosynthesis; Academic Press Inc Elsevier Science; Analytical Biochemistry; 495; 2-2016; 1-2 | |
dc.identifier | 0003-2697 | |
dc.identifier | http://hdl.handle.net/11336/52370 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1864929 | |
dc.description.abstract | Lipid-based transfection reagents are widely used for delivery of small interfering RNA into cells. We examined whether the commonly used commercial transfection reagents DharmaFECT-4 and Lipofectamine 2000 can interfere with lipid metabolism by studying cholesterogenesis. Cholesterol de novo synthesis from [14C]acetate was assessed in human hepatocyte-derived Huh-7 cells. The results revealed that DharmaFECT, but not Lipofectamine, markedly inhibited cholesterol biosynthesis by approximately 70%. Cell viability was not significantly altered. These findings suggest that caution is required in the choice of certain lipid-based transfection reagents for gene silencing experiments, particularly when assessing cholesterol metabolism. | |
dc.language | eng | |
dc.publisher | Academic Press Inc Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ab.2015.11.008 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003269715005345 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | CHOLESTEROL | |
dc.subject | HUH-7 CELLS | |
dc.subject | TRANSFECTION REAGENTS | |
dc.title | Lipid-based transfection reagents can interfere with cholesterol biosynthesis | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |