dc.creator | Gil Lorenzo, Andrea Fernanda | |
dc.creator | Bocanegra, María Victoria | |
dc.creator | Benardon, María Eugenia | |
dc.creator | Cacciamani, Valeria | |
dc.creator | Vallés, Patricia G. | |
dc.date.accessioned | 2017-03-28T17:46:44Z | |
dc.date.accessioned | 2018-11-06T12:14:39Z | |
dc.date.available | 2017-03-28T17:46:44Z | |
dc.date.available | 2018-11-06T12:14:39Z | |
dc.date.created | 2017-03-28T17:46:44Z | |
dc.date.issued | 2013-06 | |
dc.identifier | Gil Lorenzo, Andrea Fernanda; Bocanegra, María Victoria; Benardon, María Eugenia; Cacciamani, Valeria; Vallés, Patricia G.; Hsp70 regulation on Nox4/p22phox and cytoskeletal integrity as an effect of losartan in vascular smooth muscle cells; Springer; Cell Stress & Chaperones.; 19; 1; 6-2013; 115-134 | |
dc.identifier | 1355-8145 | |
dc.identifier | http://hdl.handle.net/11336/14404 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1864734 | |
dc.description.abstract | A series of signaling cascades are activated after angiotensin II binds to angiotensin II type I receptor (AT1R), a peptide that is an important mediator of oxidative stress. Hsp70 regulates a diverse set of signaling pathways through interactions with proteins. Here, we tested the hypothesis of angiotensin II AT1R inhibition effect on Hsp70 interaction with Nox4/p22phox complex and Hsp70 leading to actin cytoskeleton modulation in spontaneously hypertensive rats (SHR) vascular smooth muscle cells (VSMCs). SHR and Wistar-Kyotto rats (VSMCs from 8 to 10 weeks) were stimulated with angiotensin II (100 nmol/L) for 15 min (AII), treated with losartan (100 nmol/L) for 90 min (L), and with losartan for 90 min plus angiotensin in the last 15 min (L + AII). Whereas SHR VSMCs exposure to angiotensin II overexpressed AT1R and Nox4 nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase and slightly downregulated caveolin-1 expression, losartan decreased AT1R protein levels and increased caveolin-1 and Hsp70 expression in SHR VSMC membranes. Immunoprecipitation and immunofluorescence confocal microscopy proved interaction and colocalization of membrane translocated Hsp70 and Nox4/p22phox. Increased levels of Hsp70 contrast with the decreased immunoprecipitation of Nox4/p22phox and RhoA in membranes from SHR VSMCs (L) vs SHR VSMCs (AII). Hsp72 depletion resulted in higher Nox4 expression and increased NADPH oxidase activity in VSMCs (L + AII) from SHR when contrasted with nontransfected VSMCs (L + AII). After Hsp72 knockdown in SHR VSMCs, losartan could not impair angiotensin II-enhanced stress fiber formation and focal adhesion assembly. In conclusion, our data showing a negative regulation of Hsp70 on Nox4/p22phox demonstrates a possible mechanism in explaining the antioxidative function joined to cytoskeletal integrity modulation within the effects of losartan in VSMCs from SHR. | |
dc.language | eng | |
dc.publisher | Springer | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://doi.dx.org/10.1007/s12192-013-0439-6 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12192-013-0439-6 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | OXIDATIVE STRESS | |
dc.subject | HEAT SHOCK PROTEINS | |
dc.subject | STRESS FIBERS | |
dc.subject | CYTOSKELETON INTEGRITY | |
dc.subject | NADPH SUBUNITS | |
dc.subject | SPONTANEOUSLY HYPERTENSIVE RATS | |
dc.subject | HYPERTENSION | |
dc.title | Hsp70 regulation on Nox4/p22phox and cytoskeletal integrity as an effect of losartan in vascular smooth muscle cells | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |