dc.creatorPeluffo, Marina Cinthia
dc.creatorBussmann, L
dc.creatorStouffer, Richard L.
dc.creatorTesone, Marta
dc.date.accessioned2017-11-23T12:28:01Z
dc.date.available2017-11-23T12:28:01Z
dc.date.created2017-11-23T12:28:01Z
dc.date.issued2006-09-01
dc.identifierPeluffo, Marina Cinthia; Bussmann, L; Stouffer, Richard L.; Tesone, Marta; Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at different stages during the natural estrous cycle; BioScientifica; Reproduction; 132; 3; 1-9-2006; 465-475
dc.identifier1470-1626
dc.identifierhttp://hdl.handle.net/11336/28810
dc.identifier1741-7899
dc.identifierCONICET Digital
dc.identifierCONICET
dc.description.abstractApoptosis is associated with the regression of the corpus luteum (CL) in many species. Since caspases play a central role in apoptosis, we studied several initiators (-2, -8, and -9) and the main effector (-3) caspase in the CL during the estrous cycle of the rat. Two different populations of CL (old and new) were identified on ovaries at estrus and diestrus II (DII). Diminished (P < 0.05) luteal progesterone content and P450scc levels suggested that functional luteolysis occurred between the new CL at DII and old CL at estrus, whereas the decline (P < 0.05) in luteal weight indicated that structural regression was occurring between old CL at estrus to DII. Immunostaining for caspase-2 in luteal and endothelial cells appeared to increase as the luteal phase progressed, peaking at DII in the old CL. However, caspase-8 and -9 immunostaining showed little change with a slight increase at estrus in the old population. Notably, caspase-3 staining appeared to peak at DII in the new CL. Enzyme activity of caspase-9 increased (P < 0.05) in the new CL at DII, followed by that of caspase-2 and -3 in old CL at estrus. Caspase-8 activity did not change at any stage. The number of apoptotic cells increased at DII in the old CL. These results suggest an important role for this protease family during early events of luteolysis in the rat estrous cycle
dc.languageeng
dc.publisherBioScientifica
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/132/3/465.short
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/rep.1.00910
dc.relationinfo:eu-repo/semantics/altIdentifier/pmid/16940287
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCaspases
dc.subjectApoptosis
dc.subjectLuteolysis
dc.subjectCorpus Luteum
dc.titleExpression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at different stages during the natural estrous cycle
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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