dc.creatorAgusti, Rosalia
dc.creatorCano, María Emilia
dc.creatorCagnoni, Alejandro
dc.creatorKovensky, Jose Eduardo
dc.creatorMuchnik, Rosa
dc.creatorUhrig, Maria Laura
dc.date.accessioned2018-04-19T17:43:00Z
dc.date.accessioned2018-11-06T12:11:06Z
dc.date.available2018-04-19T17:43:00Z
dc.date.available2018-11-06T12:11:06Z
dc.date.created2018-04-19T17:43:00Z
dc.date.issued2016-10
dc.identifierAgusti, Rosalia; Cano, María Emilia; Cagnoni, Alejandro; Kovensky, Jose Eduardo; Muchnik, Rosa; et al.; Multivalent sialylation of beta-thio-glycoclusters by Trypanosoma cruzi trans sialidase and analysis by High Performance Anion Exchange Chromatography; Springer; Glycoconjugate Journal; 33; 5; 10-2016; 809-818
dc.identifier0282-0080
dc.identifierhttp://hdl.handle.net/11336/42685
dc.identifier1573-4986
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1864043
dc.description.abstractThe synthesis of multivalent sialylated glycoclusters is herein addressed by a chemoenzymatic approach using the trans-sialidase of Trypanosoma cruzi (TcTS). Multivalent β-thio-galactopyranosides and β-thio-lactosides were used as acceptor substrates and 3′-sialyllactose as the sialic acid donor. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was shown to be an excellent technique for the analysis of the reaction products. Different eluting conditions were optimized to allow the simultaneous resolution of the sialylated species, as well as their neutral precursors. The TcTS efficiently transferred sialyl residues to di, tri, tetra and octa β-thiogalactosides. In the case of an octavalent thiolactoside, up to six polysialylated compounds could be resolved. Preparative sialylation reactions were performed using the tetravalent and octavalent acceptor substrates. The main sialylated derivatives could be unequivocally assigned by MALDI mass spectrometry. Inhibition of the transfer to the natural substrate, N-acetyllactosamine, was also studied. The octalactoside caused 82 % inhibition of sialic acid transfer when we used equimolar concentrations of donor, acceptor and inhibitor.
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10719-016-9676-0
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10719-016-9676-0
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectMULTIVALENT LIGANDS
dc.subjectSIALIC ACID
dc.subjectTRANSSIALIDASE
dc.subjectTRYPANOSOMA CRUZI
dc.titleMultivalent sialylation of beta-thio-glycoclusters by Trypanosoma cruzi trans sialidase and analysis by High Performance Anion Exchange Chromatography
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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