dc.creator | Ternette, Nicola | |
dc.creator | Yang, Hongbing | |
dc.creator | Partridge, Thomas | |
dc.creator | Llano, Anuska | |
dc.creator | Cedeño, Samandhy | |
dc.creator | Fischer, Roman | |
dc.creator | Charles, Philip D. | |
dc.creator | Dudek, Nadine L. | |
dc.creator | Mothe, Beatriz | |
dc.creator | Crespo, Manuel | |
dc.creator | Fischer, William M. | |
dc.creator | Korber, Bette T. M. | |
dc.creator | Nielsen, Morten | |
dc.creator | Borrow, Persephone | |
dc.creator | Purcell, Anthony W. | |
dc.creator | Brander, Christian | |
dc.creator | Dorrell, Lucy | |
dc.creator | Kessler, Benedikt M. | |
dc.creator | Hanke, Tomáš | |
dc.date.accessioned | 2018-07-27T22:07:50Z | |
dc.date.accessioned | 2018-11-06T12:10:04Z | |
dc.date.available | 2018-07-27T22:07:50Z | |
dc.date.available | 2018-11-06T12:10:04Z | |
dc.date.created | 2018-07-27T22:07:50Z | |
dc.date.issued | 2016-01 | |
dc.identifier | Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69 | |
dc.identifier | 0014-2980 | |
dc.identifier | http://hdl.handle.net/11336/53352 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1863860 | |
dc.description.abstract | Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. | |
dc.language | eng | |
dc.publisher | Wiley VCH Verlag | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/eji.201545890 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/ | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | CYTOTOXIC T CELLS | |
dc.subject | HUMAN IMMUNODEFICIENCY VIRUS TYPE I | |
dc.subject | HUMAN LEUKOCYTE ANTIGEN | |
dc.subject | IMMUNOPEPTIDOME | |
dc.subject | MASS SPECTROMETRY | |
dc.title | Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |