dc.creatorTascon, Marcos
dc.creatorBenavente Moreno, Fernando J.
dc.creatorSanz Nebot, Victoria
dc.creatorGagliardi, Leonardo Gabriel
dc.date.accessioned2017-03-10T21:07:41Z
dc.date.accessioned2018-11-06T12:08:33Z
dc.date.available2017-03-10T21:07:41Z
dc.date.available2018-11-06T12:08:33Z
dc.date.created2017-03-10T21:07:41Z
dc.date.issued2015-03
dc.identifierTascon, Marcos; Benavente Moreno, Fernando J. ; Sanz Nebot, Victoria; Gagliardi, Leonardo Gabriel; A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis; Elsevier Science; Analytica Chimica Acta; 863; 3-2015; 78-85
dc.identifier0003-2670
dc.identifierhttp://hdl.handle.net/11336/13741
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1863546
dc.description.abstractA novel high performance system to control the temperature of the microcartridge in on-line solid phase extraction capillary electrophoresis (SPE–CE) is introduced. The mini-device consists in a thermostatic bath that fits inside of the cassette of any commercial CE instrument, while its temperature is controlled from an external circuit of liquid connecting three different water baths. The circuits are controlled from a switchboard connected to an array of electrovalves that allow to rapidly alternate the water circulation through the mini-thermostatic-bath between temperatures from 5 to 90 °C. The combination of the mini-device and the forced-air thermostatization system of the commercial CE instrument allows to optimize independently the temperature of the sample loading, the clean-up, the analyte elution and the electrophoretic separation steps. The system is used to study the effect of temperature on the C18-SPE–CE analysis of the opioid peptides, Dynorphin A (Dyn A), Endomorphin1 (END) and Met-enkephalin (MET), in both standard solutions and in spiked plasma samples. Extraction recoveries demonstrated to depend, with a non-monotonous trend, on the microcartridge temperature during the sample loading and became maximum at 60 °C. Results prove the potential of temperature control to further enhance sensitivity in SPE–CE when analytes are thermally stable.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aca.2014.12.053
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003267014014913
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCapillary electrophoresis
dc.subjectOn-line pre-concentration
dc.subjectOpioid peptides
dc.subjectSolid-Phase extraction
dc.titleA high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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