Artículos de revistas
Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
Fecha
2012-04-11Registro en:
Britos, Claudia Noelia; Cappa, Valeria Alejandra; Rivero, Cintia Wanda; Sambeth, Jorge Enrique; Lozano, Mario Enrique; et al.; Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria; Elsevier Science; Journal of Molecular Catalysis B: Enzymatic; 79; 11-4-2012; 49-53
1381-1177
CONICET Digital
CONICET
Autor
Britos, Claudia Noelia
Cappa, Valeria Alejandra
Rivero, Cintia Wanda
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge Abel
Resumen
An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB). Lactobacillus animalis ATCC 35046 showed a yield of 95% at 0.5 h to synthesize 5-fluorouracil-2-deoxyriboside (floxuridine). Calcium alginate was the best matrix
for whole-cell immobilization by entrapment. Its productivity was 87 mg/L h in a continuous bioprocess. When adsorption techniques were evaluated, DEAE-Sepharose was the support which showed higher microbial load, its productivity being 53 mg/L h. Additionally, this microorganism was able to produce 5-bromouracil-2-deoxyriboside, 6-chloropurine-2-deoxyriboside and 6-bromopurine-2
-deoxyriboside.© 2