dc.creatorValle, Lorena
dc.creatorAbatedaga, Inés
dc.creatorMoran Vieyra, Faustino Eduardo
dc.creatorBortolotti, Ana
dc.creatorCortez, Nestor Ricardo
dc.creatorBorsarelli, Claudio Darío
dc.date.accessioned2017-03-01T20:59:43Z
dc.date.available2017-03-01T20:59:43Z
dc.date.created2017-03-01T20:59:43Z
dc.date.issued2015-03
dc.identifierValle, Lorena; Abatedaga, Inés; Moran Vieyra, Faustino Eduardo; Bortolotti, Ana; Cortez, Nestor Ricardo; et al.; Enhancement of Photophysical and Photosensitizing properties of Flavin Adenine Dinucleotide by Mutagenesis of the C-terminal extension of a bacterial Flavodoxin Reductase; Wiley Vch Verlag; Chemphyschem; 16; 4; 3-2015; 872–883
dc.identifierhttp://hdl.handle.net/11336/13439
dc.identifier1439-7641
dc.description.abstractThe role of the mobile C-terminal extension present in Rhodobacter capsulatus ferredoxin–NADP(H) reductase (RcFPR) was evaluated using steady-state and dynamic spectroscopies for both intrinsic Trp and FAD in a series of mutants in the absence of NADP(H). Deletion of the six C-terminal amino acids beyond Ala266 was combined with the replacement A266Y to emulate the structure of plastidic reductases. Our results show that these modifications of the wild-type RcFPR produce subtle global conformational changes, but strongly reduce the local rigidity of the FAD-binding pocket, exposing the isoalloxazine ring to the solvent. Thus, the ultrafast charge-transfer quenching of 1 FAD* by the conserved Tyr66 residue was absent in the mutant series, producing enhancement of the excited singletand triplet-state properties of FAD. This work highlights the delicate balance of the specific interactions between FAD and the surrounding amino acids, and how the functionality and/or photostability of redox flavoproteins can be modified.
dc.languageeng
dc.publisherWiley Vch Verlag
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cphc.201402774
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cphc.201402774/abstract
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFlavoprotein
dc.subjectFluorescence
dc.subjectOxidoreductase
dc.subjectSinglet Oxygen
dc.titleEnhancement of Photophysical and Photosensitizing properties of Flavin Adenine Dinucleotide by Mutagenesis of the C-terminal extension of a bacterial Flavodoxin Reductase
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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