dc.creator | Valle, Lorena | |
dc.creator | Abatedaga, Inés | |
dc.creator | Moran Vieyra, Faustino Eduardo | |
dc.creator | Bortolotti, Ana | |
dc.creator | Cortez, Nestor Ricardo | |
dc.creator | Borsarelli, Claudio Darío | |
dc.date.accessioned | 2017-03-01T20:59:43Z | |
dc.date.available | 2017-03-01T20:59:43Z | |
dc.date.created | 2017-03-01T20:59:43Z | |
dc.date.issued | 2015-03 | |
dc.identifier | Valle, Lorena; Abatedaga, Inés; Moran Vieyra, Faustino Eduardo; Bortolotti, Ana; Cortez, Nestor Ricardo; et al.; Enhancement of Photophysical and Photosensitizing properties of Flavin Adenine Dinucleotide by Mutagenesis of the C-terminal extension of a bacterial Flavodoxin Reductase; Wiley Vch Verlag; Chemphyschem; 16; 4; 3-2015; 872–883 | |
dc.identifier | http://hdl.handle.net/11336/13439 | |
dc.identifier | 1439-7641 | |
dc.description.abstract | The role of the mobile C-terminal extension present in Rhodobacter capsulatus ferredoxin–NADP(H) reductase (RcFPR) was evaluated using steady-state and dynamic spectroscopies for both intrinsic Trp and FAD in a series of mutants in the absence of NADP(H). Deletion of the six C-terminal amino acids beyond Ala266 was combined with the replacement A266Y to emulate the structure of plastidic reductases. Our results show that these modifications of the wild-type RcFPR produce subtle global conformational changes, but strongly reduce the local rigidity of the FAD-binding pocket, exposing the isoalloxazine ring to the solvent. Thus, the ultrafast charge-transfer quenching of 1 FAD* by the conserved Tyr66 residue was absent in the mutant series, producing enhancement of the excited singletand triplet-state properties of FAD. This work highlights the delicate balance of the specific interactions between FAD and the surrounding amino acids, and how the functionality and/or photostability of redox flavoproteins can be modified. | |
dc.language | eng | |
dc.publisher | Wiley Vch Verlag | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cphc.201402774 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cphc.201402774/abstract | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Flavoprotein | |
dc.subject | Fluorescence | |
dc.subject | Oxidoreductase | |
dc.subject | Singlet Oxygen | |
dc.title | Enhancement of Photophysical and Photosensitizing properties of Flavin Adenine Dinucleotide by Mutagenesis of the C-terminal extension of a bacterial Flavodoxin Reductase | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |