dc.creatorRaiger Iustman, Laura Judith
dc.creatorRuiz, Jimena
dc.date.accessioned2018-09-26T15:44:21Z
dc.date.accessioned2018-11-06T11:59:01Z
dc.date.available2018-09-26T15:44:21Z
dc.date.available2018-11-06T11:59:01Z
dc.date.created2018-09-26T15:44:21Z
dc.date.issued2008-07
dc.identifierRaiger Iustman, Laura Judith; Ruiz, Jimena; The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 284; 2; 7-2008; 218-224
dc.identifier0378-1097
dc.identifierhttp://hdl.handle.net/11336/60880
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1861839
dc.description.abstractTo determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
dc.languageeng
dc.publisherWiley Blackwell Publishing, Inc
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1574-6968.2008.01203.x
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/284/2/218/516492
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPOLYHYDROXYALKANOATE
dc.subjectPSEUDOMONAS PUTIDA
dc.subjectREGULATION
dc.subjectRPOS
dc.subjectSTRESS RESISTANCE
dc.titleThe alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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