dc.creator | Eguílaz Rubio, Marcos | |
dc.creator | Villalonga, Reynaldo | |
dc.creator | Pingarrón, José M. | |
dc.creator | Ferreyra, Nancy Fabiana | |
dc.creator | Rivas, Gustavo Adolfo | |
dc.date.accessioned | 2018-05-08T15:02:57Z | |
dc.date.accessioned | 2018-11-06T11:53:28Z | |
dc.date.available | 2018-05-08T15:02:57Z | |
dc.date.available | 2018-11-06T11:53:28Z | |
dc.date.created | 2018-05-08T15:02:57Z | |
dc.date.issued | 2015-04 | |
dc.identifier | Eguílaz Rubio, Marcos; Villalonga, Reynaldo; Pingarrón, José M.; Ferreyra, Nancy Fabiana; Rivas, Gustavo Adolfo; Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 216; 4-2015; 629-637 | |
dc.identifier | 0925-4005 | |
dc.identifier | http://hdl.handle.net/11336/44414 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1860674 | |
dc.description.abstract | We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)2) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 ± 0.03) mA M−1 (28.6 mA M−1 cm−2), with a linear range between 2.50 × 10−4 M and 5.00 × 10−3 M, a detection limit of 0.8 μM and a quantification limit of 2.4 μM. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 °C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)2 and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)2 allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples. | |
dc.language | eng | |
dc.publisher | Elsevier Science Sa | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://www.sciencedirect.com/science/article/pii/S0925400515004827 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2015.03.112 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | BAMBOO-LIKE MULTIWALLED CARBON NANOTUBES | |
dc.subject | CARBON NANOTUBES DISPERSION | |
dc.subject | POLYETHYLENIMINE | |
dc.subject | GLUCOSE OXIDASE | |
dc.subject | BORONIC ACID | |
dc.subject | GOLD NANOPARTICLES | |
dc.subject | AMPEROMETRIC GLUCOSE BIOSENSOR | |
dc.title | Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |