info:eu-repo/semantics/article
Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
Fecha
2011-08-31Registro en:
Chumbimuni Torres, Karin Y.; Coronado, Ramon E.; Mfuh, Adelphe M.; Castro Guerrero, Carlos; Silva, María Fernanda; et al.; Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells; Royal Society of Chemistry; RSC Advances; 1; 4; 31-8-2011; 706-714
2046-2069
CONICET Digital
CONICET
Autor
Chumbimuni Torres, Karin Y.
Coronado, Ramon E.
Mfuh, Adelphe M.
Castro Guerrero, Carlos
Silva, María Fernanda
Negrete, George R.
Bizios, Rena
Garcia, Carlos D.
Resumen
This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.