info:eu-repo/semantics/article
N-nitrosomelatonin enhances photic synchronization of mammalian circadian rhythms
Fecha
2013-12Registro en:
Baidanoff, Fernando Martín; Plano, Santiago Andrés; Doctorovich, Fabio; Suárez, Sebastian; Golombek, Diego Andrés; et al.; N-nitrosomelatonin enhances photic synchronization of mammalian circadian rhythms; Wiley; Journal of Neurochemistry; 129; 1; 12-2013; 60-71
0022-3042
CONICET Digital
CONICET
Autor
Baidanoff, Fernando Martín
Plano, Santiago Andrés
Doctorovich, Fabio
Suárez, Sebastian
Golombek, Diego Andrés
Chiesa, Juan José
Resumen
Most physiological processes in mammals are synchronized to the daily light:dark cycle by a circadian clock located in the hypothalamic suprachiasmatic nucleus. Signal transduction of light-induced phase advances of the clock is mediated through a neuronal nitric oxide synthase-guanilyl cyclase pathway. We have employed a novel nitric oxide-donor, N-nitrosomelatonin, to enhance the photic synchronization of circadian rhythms in hamsters. The intraperitoneal administration of this drug before a sub-saturating light pulse at circadian time 18 generated a twofold increase of locomotor rhythm phase-advances, having no effect over saturating light pulses. This potentiation was also obtained even when inhibiting suprachiasmatic nitric oxide synthase activity. However, N-nitrosomelatonin had no effect on light-induced phase delays at circadian time 14. The photicenhancing effects were correlated with an increased suprachiasmatic immunoreactivity of FBJ murine osteosarcoma viral oncogene and period1. Moreover, in vivo nitric oxide release by N-nitrosomelatonin was verified by measuring nitrate and nitrite levels in suprachiasmatic nuclei homogenates. The compound also accelerated resynchronization to an abrupt 6-h advance in the light:dark cycle (but not resynchronization to a 6-h delay). Here, we demonstrate the chronobiotic properties of N-nitrosomelatonin, emphasizing the importance of nitric oxide-mediated transduction for circadian phase advances.