dc.creatorJeong, Yeon Tae
dc.creatorRossi, Mario
dc.creatorCermak, Lukas
dc.creatorSaraf, Anita
dc.creatorFlorens, Laurence
dc.creatorWashburn, Michael P.
dc.creatorSung, Patrick
dc.creatorSchildkraut, Carl
dc.creatorPagano, Michele
dc.date.accessioned2017-02-01T15:42:05Z
dc.date.accessioned2018-11-06T11:35:23Z
dc.date.available2017-02-01T15:42:05Z
dc.date.available2018-11-06T11:35:23Z
dc.date.created2017-02-01T15:42:05Z
dc.date.issued2013-01
dc.identifierJeong, Yeon Tae; Rossi, Mario; Cermak, Lukas; Saraf, Anita; Florens, Laurence; et al.; FBH1 mediates DNA double strand breakage and apoptosis in response to DNA replication stress; Rockefeller Univ Press; Journal Of Cell Biology; 200; 2; 1-2013; 141-149
dc.identifier0021-9525
dc.identifierhttp://hdl.handle.net/11336/12304
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1855248
dc.description.abstractProper resolution of stalled replication forks is essential for genome stability. Purification of FBH1, a UvrD DNA helicase, identified a physical interaction with replication protein A (RPA), the major cellular singlestranded DNA (ssDNA)?binding protein complex. Compared with control cells, FBH1-depleted cells responded to replication stress with considerably fewer double-strand breaks (DSBs), a dramatic reduction in the activation of<br />ATM and DNA-PK and phosphorylation of RPA2 and p53, and a significantly increased rate of survival. A minor decrease in ssDNA levels was also observed. All these phenotypes were rescued by wild-type FBH1, but not a FBH1 mutant lacking helicase activity. FBH1 depletion had no effect on other forms of genotoxic stress in which DSBs form by means that do not require ssDNA intermediates. In response to catastrophic genotoxic stress,  apoptosis prevents the persistence and propagation of DNA lesions. Our findings show that FBH1 helicase activity is required for the efficient induction of DSBs and apoptosis specifically in response to DNA replication stress.
dc.languageeng
dc.publisherRockefeller Univ Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/200/2/141.long
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549964/
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1083/jcb.201209002
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectUbiquitin
dc.subjectReplication
dc.subjectHydroxyurea
dc.subjectreplication protein A
dc.titleFBH1 mediates DNA double strand breakage and apoptosis in response to DNA replication stress
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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