dc.creator | Motiño, Omar | |
dc.creator | Agra, Noelia | |
dc.creator | Brea Contreras, Rocío | |
dc.creator | Domínguez Moreno, Marina | |
dc.creator | García Monzón, Carmelo | |
dc.creator | Vargas Castrillón, Javier | |
dc.creator | Carnovale, Cristina Ester | |
dc.creator | Boscá, Lisardo | |
dc.creator | Casado, Marta | |
dc.creator | Mayoral, Rafael | |
dc.creator | Valdecantos, M. Pilar | |
dc.creator | Valverde, Ángela M. | |
dc.creator | Frances, Daniel Eleazar Antonio | |
dc.creator | Martin Sanz, Paloma | |
dc.date.accessioned | 2018-07-19T17:34:14Z | |
dc.date.accessioned | 2018-11-06T11:33:59Z | |
dc.date.available | 2018-07-19T17:34:14Z | |
dc.date.available | 2018-11-06T11:33:59Z | |
dc.date.created | 2018-07-19T17:34:14Z | |
dc.date.issued | 2016-09 | |
dc.identifier | Motiño, Omar; Agra, Noelia; Brea Contreras, Rocío; Domínguez Moreno, Marina; García Monzón, Carmelo; et al.; Cyclooxygenase-2 expression in hepatocytes attenuates non-alcoholic steatohepatitis and liver fibrosis in mice; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1862; 9; 9-2016; 1710-1723 | |
dc.identifier | 0925-4439 | |
dc.identifier | http://hdl.handle.net/11336/52641 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1854857 | |
dc.description.abstract | Cyclooxygenase-2 (COX-2) is involved in different liver diseases but little is known about the significance of COX-2 in the development and progression of non-alcoholic steatohepatitis (NASH). This study was designed to elucidate the role of COX-2 expression in hepatocytes in the pathogenesis of steatohepatitis and hepatic fibrosis. In the present work, hepatocyte-specific COX-2 transgenic mice (hCOX-2-Tg) and their wild-type (Wt) littermates were either fed methionine-and-choline deficient (MCD) diet to establish an experimental non-alcoholic steatohepatitis (NASH) model or injected with carbon tetrachloride (CCl4) to induce liver fibrosis. In our animal model, hCOX-2-Tg mice fed MCD diet showed lower grades of steatosis, ballooning and inflammation than Wt mice, in part by reduced recruitment and infiltration of hepatic macrophages, with a corresponding decrease in serum levels of pro-inflammatory cytokines. Furthermore, hCOX-2-Tg mice showed a significant attenuation of the MCD diet-induced increase in oxidative stress and hepatic apoptosis observed in Wt mice. Even more, hCOX-2-Tg mice treated with CCl4 had significantly lower stages of fibrosis and less hepatic content of collagen, hydroxyproline and pro-fibrogenic markers than Wt controls. Collectively, our data indicates that constitutive hepatocyte COX-2 expression ameliorates NASH and liver fibrosis development in mice by reducing inflammation, oxidative stress and apoptosis and by modulating activation of hepatic stellate cells, respectively, suggesting a possible protective role for COX-2 induction in NASH/NAFLD progression. | |
dc.language | eng | |
dc.publisher | Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbadis.2016.06.009 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092544391630151X | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | COX-2 | |
dc.subject | FIBROSIS | |
dc.subject | INFLAMMATION | |
dc.subject | LIVER | |
dc.subject | STEATOHEPATITIS | |
dc.title | Cyclooxygenase-2 expression in hepatocytes attenuates non-alcoholic steatohepatitis and liver fibrosis in mice | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |