dc.creatorPena, Liliana Beatriz
dc.creatorMendez, Andrea Analia Elena
dc.creatorMatayoshi, Carolina Lucila
dc.creatorZawoznik, Myriam Sara
dc.creatorGallego, Susana Mabel
dc.date.accessioned2017-06-09T17:45:28Z
dc.date.accessioned2018-11-06T11:32:14Z
dc.date.available2017-06-09T17:45:28Z
dc.date.available2018-11-06T11:32:14Z
dc.date.created2017-06-09T17:45:28Z
dc.date.issued2015-02
dc.identifierPena, Liliana Beatriz; Mendez, Andrea Analia Elena; Matayoshi, Carolina Lucila; Zawoznik, Myriam Sara; Gallego, Susana Mabel; Early response of wheat seminal roots growing under copper excess; Elsevier Masson; Plant Physiology And Biochemistry; 87; 2-2015; 115-123
dc.identifier0981-9428
dc.identifierhttp://hdl.handle.net/11336/17889
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1854431
dc.description.abstractGrowth reduction caused by copper excess during plant photoautotrophic metabolism has been widely investigated, but information regarding early responses of root apical meristem (RAM) to toxic concentrations of this metal at the initial heterotrophic stage is certainly scarce. We analysed some determinants of seminal root growth in developing wheat seedlings germinated in the presence of 1, 5 and 10 μM CuCl2, focussing on oxidative damage to cell membrane and to proteins, and investigated the expression patterns of some genes relevant to cell cycle progression and cell expansion. The proliferation zone of the RAM was shorter under 5 and 10 μM CuCl2. Cyclin D and CDKA levels remained unchanged in the root apexes of wheat seedlings grown under these Cu2+ concentrations, but more carbonylated levels of both proteins and less ubiquitinated-cyclin D was detected under 10 μM CuCl2. Increased levels of ROS were revealed by fluorescent probes at this Cu2+ dose, and severe cell membrane damage took place at 5 and 10 μM CuCl2. Several genes related to retinoblastome phosphorylation and therefore involved in the transition from G1 to S cell cycle stage were found to be downregulated at 10 μM CuCl2, while most expansin genes here analysed were upregulated, even at a non-toxic concentration of 1 μM. These results together with previous findings suggest that a “common” signal which involves oxidative posttranslational modifications of specific cell cycle proteins may be necessary to induce root growth arrest under Cd2+ and Cu2+ stress.
dc.languageeng
dc.publisherElsevier Masson
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942814003957
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2014.12.021
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCopper
dc.subjectGrowth
dc.subjectProtein oxidation
dc.subjectTriticum aestivum L.
dc.titleEarly response of wheat seminal roots growing under copper excess
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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