dc.creator | Aguilera, Andrea Carolina | |
dc.creator | Boschin, Verónica | |
dc.creator | Carvelli, Flavia Lorena | |
dc.creator | Cavicchia, Juan Carlos | |
dc.creator | Sosa Escudero, Miguel Angel | |
dc.date.accessioned | 2018-06-19T18:11:50Z | |
dc.date.accessioned | 2018-11-06T11:28:42Z | |
dc.date.available | 2018-06-19T18:11:50Z | |
dc.date.available | 2018-11-06T11:28:42Z | |
dc.date.created | 2018-06-19T18:11:50Z | |
dc.date.issued | 2016-11 | |
dc.identifier | Aguilera, Andrea Carolina; Boschin, Verónica; Carvelli, Flavia Lorena; Cavicchia, Juan Carlos; Sosa Escudero, Miguel Angel; Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 117; 11-2016; 2464–2472 | |
dc.identifier | 0730-2312 | |
dc.identifier | http://hdl.handle.net/11336/49355 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1852879 | |
dc.description.abstract | Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-β-D-glucosaminidase and β-glucuronidase with spermatozoa appeared to be stronger. On the other hand, α-mannosidase and α-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-β-D-glucosaminidase, β-galactosidase, and β-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent-mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent-mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-β-D-glucosaminidase and β-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent-mannose-6-phosphate receptor, while β-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464–2472, 2016. | |
dc.language | eng | |
dc.publisher | Wiley-liss, Div John Wiley & Sons Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcb.25538 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.25538 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | BULL EPIDIDYMIS | |
dc.subject | LYSOSOMAL ENZYMES | |
dc.subject | MALE REPRODUCTIVE TRACT | |
dc.subject | MANNOSE-6-PHOSPHATE RECEPTORS | |
dc.subject | SPERMATOZOA | |
dc.title | Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |