info:eu-repo/semantics/article
Neonatal exposure to a glyphosate-based herbicide alters uterine decidualization in rats
Fecha
2017-10Registro en:
Ingaramo, Paola Inés; Varayoud, Jorgelina Guadalupe; Milesi, Maria Mercedes; Guerrero Schimpf, Marlise Luciana; Alarcón, Ramiro; et al.; Neonatal exposure to a glyphosate-based herbicide alters uterine decidualization in rats; Pergamon-Elsevier Science Ltd; Reproductive Toxicology; 73; 10-2017; 87-95
0890-6238
CONICET Digital
CONICET
Autor
Ingaramo, Paola Inés
Varayoud, Jorgelina Guadalupe
Milesi, Maria Mercedes
Guerrero Schimpf, Marlise Luciana
Alarcón, Ramiro
Muñoz de Toro, Monica Milagros
Luque, Enrique Hugo
Resumen
We investigated whether defective modulation of uterine signaling may cause decidualization failure in rats neonatally exposed to a glyphosate-based herbicide (GBH). Female pups received vehicle or 2 mg/kg of GBH from postnatal day (PND) 1 to PND7. On PND8 and PND21, Wnt5a and β-catenin expression was evaluated in uterine samples. On gestational day (GD) 9, Wnt5a, Wnt7a and β-catenin expression and Dkk1 and sFRP4 mRNA were evaluated on implantation sites. On PND8, GBH-exposed rats showed increased Wnt5a and β-catenin expression in luminal epithelium (LE), whereas on PND21, they showed increased Wnt5a and β-catenin expression in subepithelial stroma but decreased β-catenin expression in glandular epithelium. On GD9, GBH-exposed rats showed decreased Wnt5a and Wnt7a expression in the antimesometrial zone and LE respectively, without changes in β-catenin expression, while Dkk1 and sFRP4 were up- and down-regulated respectively. We concluded that neonatal GBH exposure may lead to embryo losses by disturbing uterine signaling.