dc.creatorWeiz, Gisela
dc.creatorBreccia, Javier Dario
dc.creatorMazzaferro, Laura
dc.date.accessioned2018-04-06T17:14:59Z
dc.date.accessioned2018-11-06T11:27:53Z
dc.date.available2018-04-06T17:14:59Z
dc.date.available2018-11-06T11:27:53Z
dc.date.created2018-04-06T17:14:59Z
dc.date.issued2017-02
dc.identifierWeiz, Gisela; Breccia, Javier Dario; Mazzaferro, Laura; Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry; Elsevier; Food Chemistry; 229; 2-2017; 44-49
dc.identifier0308-8146
dc.identifierhttp://hdl.handle.net/11336/41132
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1852460
dc.description.abstractRutin is a plant flavonoid constituted by the flavonol quercetin 3-O-linked to the disaccharide rutinose (quercetin 3-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)). The enzymatic deglycosylation of rutin is usually assessed by means of the standard method for flavonoid quantification (high performance liquid chromatography, HPLC). In this work, we have developed a spectrophotometric method for the quantification of the released quercetin. After the enzymatic reaction, quercetin is extracted with ethyl acetate, and subsequently oxidized under basic conditions. The absorbance of quercetin autooxidation products at 320 nm was correlated with the quercetin concentration by linear regression (molar extinction coefficient 26.5 (± 0.3) × 103 M-1 cm-1). The convenience of this method relies on the enzymatic activity quantification using the natural substrate by UV-visible spectrometry. Moreover, the simplicity and speed of analysis allows its application for the assay of a large number of samples.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.foodchem.2017.02.029
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814617302170
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGLUCOSIDASE
dc.subjectRHAMNOSIDASE
dc.subjectRUTIN DEGRADING ENZYMES
dc.subjectNARINGINASE
dc.titleScreening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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