info:eu-repo/semantics/article
Neurons born in the adult dentate gyrus form functional synapses with target cells
Fecha
2008Registro en:
Toni, Nicolas; Laplagne, Diego Andres; Zhao, Chunmei; Lombardi, María Gabriela; Ribak, Charles E.; et al.; Neurons born in the adult dentate gyrus form functional synapses with target cells; Nature Publishing Group; Nature Neuroscience.; 11; 8; 2008; 901-907
1097-6256
1546-1726
CONICET Digital
CONICET
Autor
Toni, Nicolas
Laplagne, Diego Andres
Zhao, Chunmei
Lombardi, María Gabriela
Ribak, Charles E.
Gage, Fred H.
Schinder, Alejandro Fabián
Resumen
Adult neurogenesis occurs in the hippocampus and the olfactory bulb of the mammalian CNS. Recent studies have demonstrated that newborn granule cells of the adult hippocampus are postsynaptic targets of excitatory and inhibitory neurons, but evidence of synapse formation by the axons of these cells is still lacking. By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, we found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed us to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices. Our structural and functional evidence indicates that axons of adult-born granule cells establish synapses with hilar interneurons, mossy cells and CA3 pyramidal cells and release glutamate as their main neurotransmitter.