dc.creatorGiambelluca, Miriam Soledad
dc.creatorGende, Oscar Alfredo
dc.date.accessioned2018-09-26T15:29:39Z
dc.date.accessioned2018-11-06T11:22:09Z
dc.date.available2018-09-26T15:29:39Z
dc.date.available2018-11-06T11:22:09Z
dc.date.created2018-09-26T15:29:39Z
dc.date.issued2011
dc.identifierGiambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-571
dc.identifier0006-291X
dc.identifierhttp://hdl.handle.net/11336/60871
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1849700
dc.description.abstractIt is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11008060
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbrc.2011.05.046
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCHLORIDE-BICARBONATE EXCHANGER
dc.subjectNEUTROPHILS
dc.subjectINTRACELULAR PH
dc.subjectCHLORIDE
dc.titleCl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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