dc.creator | Giambelluca, Miriam Soledad | |
dc.creator | Gende, Oscar Alfredo | |
dc.date.accessioned | 2018-09-26T15:29:39Z | |
dc.date.accessioned | 2018-11-06T11:22:09Z | |
dc.date.available | 2018-09-26T15:29:39Z | |
dc.date.available | 2018-11-06T11:22:09Z | |
dc.date.created | 2018-09-26T15:29:39Z | |
dc.date.issued | 2011 | |
dc.identifier | Giambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-571 | |
dc.identifier | 0006-291X | |
dc.identifier | http://hdl.handle.net/11336/60871 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1849700 | |
dc.description.abstract | It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils. | |
dc.language | eng | |
dc.publisher | Academic Press Inc Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11008060 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbrc.2011.05.046 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | CHLORIDE-BICARBONATE EXCHANGER | |
dc.subject | NEUTROPHILS | |
dc.subject | INTRACELULAR PH | |
dc.subject | CHLORIDE | |
dc.title | Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |