Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts

dc.creatorMorcillo, Nora S.
dc.creatorImperiale, Belén Rocío
dc.creatorDi Giulio, Angela
dc.creatorZumárraga, Martín José
dc.creatorTakiff, Howard
dc.creatorCataldi, Ángel Adrián
dc.date2014-06
dc.identifierhttp://hdl.handle.net/11336/35740
dc.identifierMorcillo, Nora S.; Imperiale, Belén Rocío; Di Giulio, Angela; Zumárraga, Martín José; Takiff, Howard; et al.; Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts; Churchill Livingstone; Tuberculosis (Edinb); 94; 6; 6-2014; 672-677
dc.identifier1472-9792
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionThere has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
dc.descriptionFil: Morcillo, Nora S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.descriptionFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.descriptionFil: Di Giulio, Angela. Hospital Petrona V. de Cordero. San Fernando; Argentina
dc.descriptionFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.descriptionFil: Takiff, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
dc.descriptionFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
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dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherChurchill Livingstone
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979214203908
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.tube.2014.08.003
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectDrug-Resistance
dc.subjectTuberculosis
dc.subjectFitness
dc.subjectHousehold Contacts
dc.subjectFamily Transmission
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleFitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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