Alkaline and thermostable polygalacturonase from Streptomyces halstedii ATCC 10897 with applications in waste waters

dc.creatorRamírez Tapias, Yuly Andrea
dc.creatorRivero, Cintia Wanda
dc.creatorBritos, Claudia Noelia
dc.creatorTrelles, Jorge Abel
dc.date2015-04
dc.identifierhttp://hdl.handle.net/11336/51013
dc.identifierRamírez Tapias, Yuly Andrea; Rivero, Cintia Wanda; Britos, Claudia Noelia; Trelles, Jorge Abel; Alkaline and thermostable polygalacturonase from Streptomyces halstedii ATCC 10897 with applications in waste waters; Elsevier; Biocatalysis and Agricultural Biotechnology; 4; 2; 4-2015; 221-228
dc.identifier1878-8181
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionPectin degrading enzymes with polygalacturonase (PG) activity hydrolyze α-(1,4) glycosidic bonds of polysaccharides present in higher plants. In the current study one hundred bacterial strains were screened for extracellular PG activity using an inductive culture medium. Optimization of fermentation conditions for Streptomyces halstedii ATCC 10897 was conducted using experimental designs. The maximum enzymatic activity obtained was 3.489. U/mL and 98.0% of viscosity reduction after 12. h of fermentation using soy peptone as unique source of carbon and nitrogen. PG from S. halstedii ATCC 10897 showed high thermal stability, an approximate molecular weight of 48. kDa and its optimum conditions for catalytic reaction were 50. °C and pH 12.0. This study reveals that alkaline PG is a useful enzyme for depectinization in alkaline pulping mill and papermaking waste waters. halstedii produces extremely alkalophilic polygalacturonase (48kDa) at 12h.
dc.descriptionFil: Ramírez Tapias, Yuly Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.descriptionFil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.descriptionFil: Britos, Claudia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.descriptionFil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcab.2014.12.004
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1878818114001546
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectCulture Medium Design
dc.subjectFermentation Optimization
dc.subjectScreening
dc.subjectWaste Waters
dc.subjecthttps://purl.org/becyt/ford/2.4
dc.subjecthttps://purl.org/becyt/ford/2
dc.titleAlkaline and thermostable polygalacturonase from Streptomyces halstedii ATCC 10897 with applications in waste waters
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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