dc.creator | Leal Denis, Maria Florencia | |
dc.creator | Incicco, Juan Jeremías | |
dc.creator | Espelt, Maria Victoria | |
dc.creator | Verstraeten, Sandra Viviana | |
dc.creator | Pignataro, Omar Pedro | |
dc.creator | Lazarowski, Eduardo R. | |
dc.creator | Schwarzbaum, Pablo Julio | |
dc.date.accessioned | 2015-11-12T19:24:37Z | |
dc.date.accessioned | 2018-11-06T11:15:10Z | |
dc.date.available | 2015-11-12T19:24:37Z | |
dc.date.available | 2018-11-06T11:15:10Z | |
dc.date.created | 2015-11-12T19:24:37Z | |
dc.date.issued | 2013-10 | |
dc.identifier | Leal Denis, Maria Florencia; Incicco, Juan Jeremías; Espelt, Maria Victoria; Verstraeten, Sandra Viviana; Pignataro, Omar Pedro; et al.; Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes; Elsevier; Biochimica et Biophysica Acta- General Subjects; 1830; 10; 10-2013; 4692-4707 | |
dc.identifier | 0304-4165 | |
dc.identifier | http://hdl.handle.net/11336/2772 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1847733 | |
dc.description.abstract | Background: The peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intraand extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics). Methods: Human erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin–luciferase based real-time luminometry.
Results: Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10 μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers. Conclusions: MST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers. General significance: Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbagen.2013.05.033 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304416513002298 | |
dc.relation | info:eu-repo/semantics/altIdentifier/issn/0304-4165 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999873/ | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | EXTRACELLULAR ATP | |
dc.subject | ATPASES | |
dc.subject | ERYTHROCYTE | |
dc.subject | PANNEXIN 1 | |
dc.title | Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |