Tubulinosema pampeana sp. n. (Microsporidia, Tubulinosematidae), a pathogen of the South American bumble bee Bombus atratus

dc.creatorPlischuk, Santiago
dc.creatorSainscrainte, Neil D.
dc.creatorBecnel, James J.
dc.creatorEstep, Alden S.
dc.creatorLange, Carlos Ernesto
dc.date2015-03
dc.identifierhttp://hdl.handle.net/11336/10455
dc.identifierPlischuk, Santiago; Sainscrainte, Neil D.; Becnel, James J.; Estep, Alden S.; Lange, Carlos Ernesto; Tubulinosema pampeana sp. n. (Microsporidia, Tubulinosematidae), a pathogen of the South American bumble bee Bombus atratus; Academic Press Inc Elsevier Science; Journal Of Invertebrate Pathology; 126; 3-2015; 31-42
dc.identifier0022-2011
dc.descriptionAn undescribed microsporidium was detected and isolated from the South American bumble bee Bombus atratus collected in the Pampas region of Argentina. Infection intensity in workers averaged 8.2 107 spores/bee. The main site of infection was adipose tissue where hypertrophy of adipocytes resulted in cyst-like body formation. Mature spores were ovoid and monomorphic. They measured 4.00 lm 2.37 lm (fresh) or 3.98 lm 1.88 lm (fixed). All stages were diplokariotic and developed in direct contact with host cytoplasm. Isofilar polar filament was arranged in 16 coils in one or, posteriorly, two layers. Coiling angle was variable, between perpendicular and almost parallel to major spore axis. Late meronts and sporogonial stages were surrounded by vesicles of approximately 60 nm in diameter. Based on both new and already designed primers, a 1827 bp (SSUrRNA, ITS, LSUrRNA) sequence was obtained. Data analyses suggest that this microsporidium is a new species of the genus Tubulinosema. The name Tubulinosema pampeana sp. n. is proposed.
dc.descriptionFil: Plischuk, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
dc.descriptionFil: Sainscrainte, Neil D.. United States Department Of Agriculture. Center For Medical Agric And Vet Entomology; Estados Unidos
dc.descriptionFil: Becnel, James J.. United States Department Of Agriculture. Center For Medical Agric And Vet Entomology; Estados Unidos
dc.descriptionFil: Estep, Alden S.. Navy Entomology Center Of Excellence, Jacksonville; . United States Department Of Agriculture. Center For Medical Agric And Vet Entomology; Estados Unidos
dc.descriptionFil: Lange, Carlos Ernesto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
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dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.01.006
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/25637516
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectArgentina
dc.subjectBee Health
dc.subjectCyst-Like Bodies
dc.subjectNosema
dc.subjectPampas Region
dc.subjectPollinator
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.subjecthttps://purl.org/becyt/ford/4.1
dc.subjecthttps://purl.org/becyt/ford/4
dc.titleTubulinosema pampeana sp. n. (Microsporidia, Tubulinosematidae), a pathogen of the South American bumble bee Bombus atratus
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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