Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors

dc.creatorPrimo, Emiliano Nicolás
dc.creatorGutierrez, Fabiana Andrea
dc.creatorRubianes, María Dolores
dc.creatorRivas, Gustavo Adolfo
dc.date2015-11-10
dc.identifierhttp://hdl.handle.net/11336/47381
dc.identifierPrimo, Emiliano Nicolás; Gutierrez, Fabiana Andrea; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Bamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 182; 10-11-2015; 391-397
dc.identifier0013-4686
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionThis work reports the successful application of bamboo-like multiwalled carbon nanotubes (bCNT) non-covalently functionalized with calf-thymus double stranded DNA (dsDNA) as a robust platform (bCNT-dsDNA) to build electrochemical biosensors. The “model system” proposed here as a proof of concept was an enzymatic biosensor devoted to glucose quantification obtained by layer-by-layer self-assemby of polydiallyldimethylammonium (PDDA) and glucose oxidase (GOx) at glassy carbon electrodes (GCE) modified with bCNT-dsDNA (GCE/bCNT-dsDNA/(PDDA/GOx)n). The influence of GOx and PDDA assembling conditions and the effect of the number of PDDA/GOx bilayers (n) on the performance of the resulting biosensor is critically discussed. The supramolecular architecture was characterized by electrochemical impedance spectroscopy from the charge transfer resistance of quinone/hydroquinone and potassium ferrocyanide/potassium ferricyanide; by cyclic voltammetry from the surface concentration of GOx using ferrocene methanol as enzyme regenerator; by amperometry from the response of the enzymatically generated hydrogen peroxide; and by surface plasmon resonance from the changes in the plasmon resonance angle. The analytical parameters obtained with GCE/bCNT-dsDNA/(PDDA/GOx)3 for the amperometric quantification of glucose at 0.700 V were: sensitivity of (265 ± 7) μA mM−1 cm−2, linear range between 0.25 and 2.50 × 10−3 M, detection limit of 50 μM, repeatability of 3.6% (n = 10), and negligible interference from maltose, galactose, fructose and manose. The biosensor was successfully used for the sensitive quantification of glucose in beverages and a medicine sample.
dc.descriptionFil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.descriptionFil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.descriptionFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.descriptionFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
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dc.languageeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0013468615304400
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.09.028
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectCarbon Nanotubes Functionalization
dc.subjectCalf-Thymus Double Stranded
dc.subjectGlucose Oxidase
dc.subjectLayer-By-Layer Biosensors
dc.subjectSelf-Assembly
dc.subjectElectrochemical Glucose Biosensor
dc.subjectBamboo-Like Multiwalled Carbon Nanotubes
dc.subjecthttps://purl.org/becyt/ford/1.4
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleBamboo-like multiwalled carbon nanotubes dispersed in double stranded calf-thymus DNA as a new analytical platform for building layer-by-layer based biosensors
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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