Argentina | info:eu-repo/semantics/article

Raf/MEK/ERK pathway activation is required for Junín virus replication

dc.creatorRodríguez, María Eugenia
dc.creatorBrunetti, Jesús Emanuel
dc.creatorWachsman, Mónica Beatriz
dc.creatorScolaro, Luis Alberto
dc.creatorCastilla, Viviana
dc.date2014-04
dc.identifierhttp://hdl.handle.net/11336/32209
dc.identifierCastilla, Viviana; Scolaro, Luis Alberto; Wachsman, Mónica Beatriz; Brunetti, Jesús Emanuel; Rodríguez, María Eugenia; Raf/MEK/ERK pathway activation is required for Junín virus replication; Society for General Microbiology; Journal of General Virology; 95; 4-2014; 799-805
dc.identifier0022-1317
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionIn the present work we investigated the importance of the Raf/MEK/ERK signalling pathway in the multiplication of the arenavirus Junı´n (JUNV) in monkey and human cell cultures. We established that JUNV induces a biphasic activation of ERK and we proved that a specific inhibitor of the ERK pathway, U0126, impairs viral replication. Furthermore, U0126 exerted inhibitory action against the arenaviruses Tacaribe and Pichinde. Moreover, treatment with known ERK activators such as phorbol 12-myristate 13-acetate and serum increased viral yields whereas ERK silencing by small interfering RNAs caused the inhibition of viral multiplication. Therefore, activation of the Raf/MEK/ ERK signalling pathway is required to ensure efficient JUNV replication and may constitute a host target for the development of novel effective therapeutic strategies to deal with arenavirus infections.
dc.descriptionFil: Rodríguez, María Eugenia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
dc.descriptionFil: Brunetti, Jesús Emanuel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Wachsman, Mónica Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
dc.descriptionFil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociety for General Microbiology
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.061242-0
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.061242-0
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectJunin Virus
dc.subjectRaf/Mek/Erk
dc.subjectCellular Pathway
dc.subjectReplication
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleRaf/MEK/ERK pathway activation is required for Junín virus replication
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución