Artículos de revistas
Validation of photodynamic action via photobleaching of a new curcumin-based composite with enhanced water solubility
Fecha
2014-09Registro en:
Journal of Fluorescence, New York : Springer, v. 24, n. 5, p. 1407-1413, Sept. 2014
1053-0509
10.1007/s10895-014-1422-z
Autor
Rego Filho, Francisco G.
Araujo, Maria T.
Oliveira, Kleber T.
Bagnato, Vanderlei Salvador
Institución
Resumen
Motivated by the photochemical and photophysical properties of curcumin-based composites, the characteristics of a new curcumin-based water-soluble salt were investigated via absorption and fluorescence spectroscopy. Photobleaching was investigated using a set of LEDs in three different wavelengths (405 nm, 450 nm and 470 nm) to illuminate an aqueous solution of curcumin, evaluating its degradation for five different exposure times (0, 5, 15, 45 and 105 minutes). The results were compared with equivalent measurements of dark degradation and illumination in the presence of a singlet-oxygen quencher. Three solution concentrations (50, 100 and 150 μg/ml) were studied. To measure the fluorescence, it was used low power 405 nm excitation laser source. Time dependent photodegradation of curcumin was observed, as compared to the natural degradation of samples maintained on a dark environment. Two main absorption peaks were detected and their relation responded to both concentration and wavelength of the illumination source. A spectral correlation between absorption of curcumin and the emission bands of the sources showed an optimal spectral overlap for the 450 nm LED. For this source, photobleaching showed a less intense degradation on the presence of singlet oxygen quencher. This last result confirmed singlet oxygen production in vitro, indicating a strong potential of this composite to be used as a bluelight- activated photosensitizer.