Invasion of Ureaplasma diversum in bovine spermatozoids

Buzinhani, Melissa; Yamaguti, Maurício; Oliveira, Rosângela C; Cortez, Beatriz A; Marques, Lucas M; Machado-Santelli, Gláucia M; Assumpção, Mayra E; Timenetsky, Jorge

Fecha

2011-10-27

Registro en:

BMC Research Notes. 2011 Oct 27;4(1):455

http://dx.doi.org/10.1186/1756-0500-4-455

http://www.producao.usp.br/handle/BDPI/48255

http://repositorioslatinoamericanos.uchile.cl/handle/2250/1643927

Autor

Buzinhani, Melissa

Yamaguti, Maurício

Oliveira, Rosângela C

Cortez, Beatriz A

Marques, Lucas M

Machado-Santelli, Gláucia M

Assumpção, Mayra E

Timenetsky, Jorge

Institución

Universidade de São Paulo (Brasil)

Resumen

Abstract Background Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay. Findings U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids. Conclusions The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.

Materias

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