dc.creator | Nakahata, Adriana M. | |
dc.creator | Suzuki, Daniela E. | |
dc.creator | Rodini, Carolina O. | |
dc.creator | Fiuza, Mayara L. | |
dc.creator | Okamoto, Oswaldo Keith | |
dc.date.accessioned | 2014-08-01T17:55:50Z | |
dc.date.accessioned | 2018-07-04T16:51:21Z | |
dc.date.available | 2014-08-01T17:55:50Z | |
dc.date.available | 2018-07-04T16:51:21Z | |
dc.date.created | 2014-08-01T17:55:50Z | |
dc.date.issued | 2014-07-22 | |
dc.identifier | Oncology Letters, Athens, v.8, p.1487-1491,2014 | |
dc.identifier | 1792-1082 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/45931 | |
dc.identifier | 10.3892/ol.2014.2369 | |
dc.identifier | http://www.spandidos-publications.com/10.3892/ol.2014.2369 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1641294 | |
dc.description.abstract | In a previous genome‑wide expression profiling study,
we identified E2F2 as a hyperexpressed gene in stem‑like cells
of distinct glioblastoma multiforme (GBM) specimens. Since
the encoded E2F2 transcription factor has been implicated in
both tumor suppression and tumor development, we conducted a
functional study to investigate the pertinence of E2F2 to human
gliomagenesis. E2F2 expression was knocked down by trans‑
fecting U87MG cells with plasmids carrying a specific silencing
shRNA. Upon E2F2 silencing, in vitro cell proliferation was
significantly reduced, as indicated by a time‑course analysis of
viable tumor cells. Anchorage‑independent cell growth was also
significantly inhibited after E2F2 silencing, based on cell colony
formation in soft agar. Subcutaneous and orthotopic xenograft
models of GBM in nude mice also indicated inhibition of tumor
development in vivo, following E2F2 silencing. As expression
of the E2F2 gene is associated with glioblastoma stem cells
and is involved in the transformation of human astrocytes, the
present findings suggest that E2F2 is involved in gliomagenesis
and could be explored as a potential therapeutic target in malig‑
nant gliomas. | |
dc.language | eng | |
dc.publisher | Spandidos Publications | |
dc.publisher | Athens | |
dc.relation | Oncology Letters | |
dc.rights | Copyright © Spandidos Publications 2014 | |
dc.rights | openAccess | |
dc.subject | E2F2 | |
dc.subject | Glioblastoma multiforme | |
dc.subject | Cancer | |
dc.subject | Tumorigenesis | |
dc.title | RNAi‑mediated knockdown of E2F2 inhibits tumorigenicity of
human glioblastoma cells | |
dc.type | Artículos de revistas | |