dc.creator | Manzine, Livia Regina | |
dc.creator | Serrão, Vitor Hugo Balasco | |
dc.creator | Lima, Luis Maurício Trambaioli da Rocha e | |
dc.creator | Souza, Marcos Michel de | |
dc.creator | Bettini, Jefferson | |
dc.creator | Portugal, Rodrigo Villares | |
dc.creator | Heel, Marin van | |
dc.creator | Thiemann, Otavio Henrique | |
dc.date.accessioned | 2014-05-23T17:43:46Z | |
dc.date.accessioned | 2018-07-04T16:45:58Z | |
dc.date.available | 2014-05-23T17:43:46Z | |
dc.date.available | 2018-07-04T16:45:58Z | |
dc.date.created | 2014-05-23T17:43:46Z | |
dc.date.issued | 2013-04 | |
dc.identifier | FEBS Letters, Amsterdam : Elsevier BV, v. 587, n. 7, p. 906-911, Apr. 2013 | |
dc.identifier | 0014-5793 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/45006 | |
dc.identifier | 10.1016/j.febslet.2013.02.014 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1640071 | |
dc.description.abstract | In bacteria selenocysteyl-tRNAsec (SelC) is synthesized by selenocysteine synthase (SelA). Here we show by fluorescence anisotropy binding assays and electron microscopical symmetry analysis that the SelA-tRNAsec binding stoichiometry is of one tRNAsec molecule per SelA monomer (1:1) rather than the 1:2 value proposed previously. Negative stain transmission electron microscopy revealed a D5 pointgroup symmetry for the SelA-tRNAsec assembly both with and without tRNAsec bound. Furthermore, SelA can associate forming a supramolecular complex of stacked decamer rings, which does not occur in the presence of tRNAsec. We discuss the structure-function relationships of these assemblies and their regulatory role in bacterial selenocysteyl-tRNAsec synthesis. | |
dc.language | eng | |
dc.publisher | Elsevier BV | |
dc.publisher | Amsterdam | |
dc.relation | FEBS Letters | |
dc.rights | Copyright Elsevier B.V. | |
dc.rights | restrictedAccess | |
dc.subject | Selenocysteine synthase | |
dc.subject | tRNAsec | |
dc.subject | Stoichiometry | |
dc.title | Assembly stoichiometry of bacterial selenocysteine synthase and SelC (tRNAsec) | |
dc.type | Artículos de revistas | |