dc.creatorTeixeira, Leandro Emidio
dc.creatorKanunfre, Kelly Aparecida
dc.creatorShimokawa, Paulo Tadashi
dc.creatorTarga, Lilia Spaleta
dc.creatorRodrigues, Jonatas Cristian
dc.creatorDomingues, Wilson
dc.creatorYamamoto, Lidia
dc.creatorOkay, Thelma Suely
dc.date.accessioned2014-03-18T11:31:53Z
dc.date.accessioned2018-07-04T16:44:39Z
dc.date.available2014-03-18T11:31:53Z
dc.date.available2018-07-04T16:44:39Z
dc.date.created2014-03-18T11:31:53Z
dc.date.issued2013
dc.identifierRev Soc Bras Med Trop, v. 46, n. 5, p. 584-588, Sep-Oct, 2013.
dc.identifierhttp://www.producao.usp.br/handle/BDPI/44170
dc.identifier10.1590/0037-8682-0095-2013
dc.identifierhttp://www.scielo.br/pdf/rsbmt/v46n5/0037-8682-rsbmt-0037-8682-0095.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1639762
dc.description.abstractIntroduction Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim of this study was to compare the performance of 4 PCR-based methods for the laboratory diagnosis of toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included in the study. The definition of cases was based on a 12-month follow-up of the infants. Methods Amniotic fluid samples were submitted to DNA extraction and amplification by the following 4 Toxoplasma techniques performed with parasite B1 gene primers: conventional PCR, nested-PCR, multiplex-nested-PCR, and real-time PCR. Seven parameters were analyzed, sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and efficiency (Ef). Results Fifty-nine of the 100 infants had toxoplasmosis; 42 (71.2%) had IgM antibodies at birth but were asymptomatic, and the remaining 17 cases had non-detectable IgM antibodies but high IgG antibody titers that were associated with retinochoroiditis in 8 (13.5%) cases, abnormal cranial ultrasound in 5 (8.5%) cases, and signs/symptoms suggestive of infection in 4 (6.8%) cases. The conventional PCR assay detected 50 cases (9 false-negatives), nested-PCR detected 58 cases (1 false-negative and 4 false-positives), multiplex-nested-PCR detected 57 cases (2 false-negatives), and real-time-PCR detected 58 cases (1 false-negative). Conclusions The real-time PCR assay was the best-performing technique based on the parameters of Se (98.3%), Sp (100%), PPV (100%), NPV (97.6%), PLR (â^ž), NLR (0.017), and Ef (99%).
dc.languageeng
dc.publisherBelo Horizonte
dc.relationRevista da Sociedade Brasileira de Medicina Tropical
dc.rightsSociedade Brasileira de Medicina Tropical
dc.rightsopenAccess
dc.subjectCongenital toxoplasmosis
dc.subjectCongenital infection
dc.subjectMolecular diagnosis
dc.subjectPCR
dc.subjectQuantitative PCR
dc.subjectReal-time PCR
dc.titleThe performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples
dc.typeArtículos de revistas


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