dc.creator | Lopes, Andressa Bolsoni | |
dc.creator | Festuccia, William Tadeu Lara | |
dc.creator | Farias, Talita da Silva Mendes de | |
dc.creator | Chimin, Patricia | |
dc.creator | Leal, Francisco Leonardo Tôrres | |
dc.creator | Derogis, Priscilla Bento Matos Cruz | |
dc.creator | Andrade, Paula Bresciani Martins de | |
dc.creator | Miyamoto, Sayuri | |
dc.creator | Lima, Fabio Bessa | |
dc.creator | Curi, Rui | |
dc.creator | Alonso-Vale, Maria Isabel C. | |
dc.date.accessioned | 2014-04-22T19:27:31Z | |
dc.date.accessioned | 2018-07-04T16:43:29Z | |
dc.date.available | 2014-04-22T19:27:31Z | |
dc.date.available | 2018-07-04T16:43:29Z | |
dc.date.created | 2014-04-22T19:27:31Z | |
dc.date.issued | 2013-11-01 | |
dc.identifier | AJP - Endocrinology and Metabolism, Bethesda, v.305, n.9, p.e1093-e1102, 2013 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/44572 | |
dc.identifier | 10.1152/ajpendo.00082.2013 | |
dc.identifier | http://dx.doi.org/10.1152/ajpendo.00082.2013 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1639495 | |
dc.description.abstract | We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16:1n7, 200 μM) or palmitic acid (16:0, 200 μM) for 24 h and primary adipocytes from wild-type or PPARα-deficient mice treated with 16:1n7 (300 mg•kg(-1)•day(-1)) or oleic acid (18:1n9, 300 mg•kg(-1)•day(-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16:0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16:1n7, which can be prevented by pharmacological inhibition of PPARα, was associated with higher rates of PPARα binding to DNA. In contrast to lipolysis, both 16:1n7 and 16:0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPARα-deficient mice with 16:1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. In contrast to lipolysis, however, 16:1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPARα-deficient mice. In conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPARα | |
dc.language | eng | |
dc.publisher | American Physiological Society | |
dc.publisher | Bethesda | |
dc.relation | AJP - Endocrinology and Metabolism | |
dc.rights | American Physiological Society | |
dc.rights | restrictedAccess | |
dc.subject | ATGL | |
dc.subject | HSL | |
dc.subject | Lipogenesis | |
dc.subject | Triacylglycerol/fatty acid cycle | |
dc.title | Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner | |
dc.type | Artículos de revistas | |