Artículos de revistas
Gene expression and phenotypic traits of Aggregatibacter actinomycetemcomitans in response to environmental changes
Fecha
2013-12Registro en:
Journal of Periodontal Research, Copenhagen, v.48, n.6, p.766-772, 2013
10.1111/jre.12067
Autor
Longo, Priscila Larcher
Nunes, A. C. R.
Umeda, J. E.
Mayer, Marcia Pinto Alves
Institución
Resumen
Background and Objective: Periodontopathogens experience several challenges in
the oral cavity that may influence their transcription profile and resulting phenotype.
This study evaluated the effect of environmental changes on phenotype and
gene expression in a serotype b Aggregatibacter actinomycetemcomitans isolate.
Material and Methods: Cultures in early exponential phase and at the start of
stationary growth phase in microaerophilic and anaerobic atmospheres were
evaluated. Cell hydrophobic properties were measured by adherence to
n-hexadecane; in addition, adhesion to, and the ability to invade, KB cells was
evaluated. Relative transcription of 12 virulence-associated genes was
determined by real-time reverse transcritption quantitative PCR.
Results: The culture conditions tested in this study were found to influence the
phenotypic and genotypic traits of A. actinomycetemcomitans. Cells cultured in
microaerophilic conditions were the most hydrophobic, reached the highest
adhesion efficiency and showed up-regulation of omp100 (which encodes an
adhesion) and pga (related to polysaccharide synthesis). Cells grown anaerobically
were more invasive to epithelial cells and showed up-regulation of genes
involved in host-cell invasion or apoptosis induction (such as apaH, omp29,
cagE and cdtB) and in adhesion to extracellular matrix protein (emaA).
Conclusion: Environmental conditions of different oral habitats may influence
the expression of factors involved in the binding of A. actinomycetemcomitans to
host tissues and the damage resulting thereby, and thus should be considered in
in-vitro studies assessing its pathogenic potential.