dc.creator | Slompo, Camila | |
dc.creator | Buzalaf, Camila P. | |
dc.creator | Damante, Carla A. | |
dc.creator | Martins, Gisele M. | |
dc.creator | Hannas, Angélica R | |
dc.creator | Buzalaf, Marília A. R | |
dc.creator | Oliveira, Rodrigo C | |
dc.date.accessioned | 2013-11-04T12:32:04Z | |
dc.date.accessioned | 2018-07-04T16:35:46Z | |
dc.date.available | 2013-11-04T12:32:04Z | |
dc.date.available | 2018-07-04T16:35:46Z | |
dc.date.created | 2013-11-04T12:32:04Z | |
dc.date.issued | 2012 | |
dc.identifier | Braz. Dent. J.,v.23,n.6,p.629-634,2012 | |
dc.identifier | 0103-6440 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/39297 | |
dc.identifier | 10.1590/S0103-64402012000600002 | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1637733 | |
dc.description.abstract | This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10-6 M, 10-5 M, 10-4 M and 10-3 M) for 24, 48, 72 and 96 h at 37ºC, 5% CO2. Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10-3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling. | |
dc.language | eng | |
dc.publisher | Fundação Odontológica de Ribeirão Preto | |
dc.relation | Brazilian Dental Journal | |
dc.rights | openAccess | |
dc.subject | metalloproteinase | |
dc.subject | phosphatase | |
dc.subject | fluoride | |
dc.title | Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities | |
dc.type | Artículos de revistas | |