dc.creatorSlompo, Camila
dc.creatorBuzalaf, Camila P.
dc.creatorDamante, Carla A.
dc.creatorMartins, Gisele M.
dc.creatorHannas, Angélica R
dc.creatorBuzalaf, Marília A. R
dc.creatorOliveira, Rodrigo C
dc.date.accessioned2013-11-04T12:32:04Z
dc.date.accessioned2018-07-04T16:35:46Z
dc.date.available2013-11-04T12:32:04Z
dc.date.available2018-07-04T16:35:46Z
dc.date.created2013-11-04T12:32:04Z
dc.date.issued2012
dc.identifierBraz. Dent. J.,v.23,n.6,p.629-634,2012
dc.identifier0103-6440
dc.identifierhttp://www.producao.usp.br/handle/BDPI/39297
dc.identifier10.1590/S0103-64402012000600002
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_pdf&pid=S0103-64402012000600002&lng=en&nrm=iso&tlng=en
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1637733
dc.description.abstractThis study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10-6 M, 10-5 M, 10-4 M and 10-3 M) for 24, 48, 72 and 96 h at 37ºC, 5% CO2. Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10-3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.
dc.languageeng
dc.publisherFundação Odontológica de Ribeirão Preto
dc.relationBrazilian Dental Journal
dc.rightsopenAccess
dc.subjectmetalloproteinase
dc.subjectphosphatase
dc.subjectfluoride
dc.titleFluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities
dc.typeArtículos de revistas


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